Shimadzu Corporation

Shoken Higashide

Masaaki Ito

Toru Ezure

Takashi Suzuki

Koki Endo

Satoko Shiraki

The present invention provides a preparation method of an insect cell extract solution for cell-free protein synthesis, the insect cell extract solution, a protein synthesis method in a cell-free system, which uses the insect cell extract solution, and a kit for cell-free protein synthesis containing the insect cell extract solution. The extract solution is easily prepared by the method of the present invention and can synthesize a higher amount of protein than by extract solutions prepared by conventional methods.

Cell-free protein synthesis method using insect cell extract solution

Preparation method of insect cell extract solution for cell-free protein synthesis, the insect cell extract solution and cell-free synthesis method of protein using the insect cell extract solution

The present invention provides a novel extract solution for cell-free protein synthesis, which contains at least an extract derived from aL. tissue and an exogenous mRNA, which is easy to prepare and which is capable of realizing cell-free protein synthesis that can synthesize glycoprotein, a cell-free protein synthesis method using it, and a method for preparing an extract solution for cell-free protein synthesis, which includes adding an exogenous mRNA to an extract derived from aL. tissue.

Extract solution for cell-free protein synthesis, method for cell-free protein synthesis using same and method for preparation of the extract solution

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Osaka, Japan

Shoken Higashide