The University of Electro-communications

Tokyo University of Pharmacy & Life Sciences

Campus Create Co., Ltd.

Shojiro Maki

Haruki Niwa

Tsuyoshi Saito

Shigeru Nishiyama

Hiroshi Aoyama

Ryosuke Ijuin

Ryohei Moriya

Nobuo Kitada

The aim is to provide a novel compound that can luminesce with high brightness and be used as a luminescent substrate in a firefly bioluminescence system. Provided is a heterocyclic compound, or salt thereof, represented by the following general formula (1): [R, R, and Rare independently hydrogen or an alkyl group having a carbon number of 1-4, with provisos that Rand Rare optionally bonded to form a ring and that one of Rand Ris optionally bonded to Yto form a ring; X is S, O, NR, or CH, and Yand Yare independently N or CR, where each Ris independently hydrogen, an alkyl group having a carbon number of 1-4, an alkenyl group having a carbon number of 2-4, or an acyl group having a carbon number of 2-4; n is an integer of 0-4].

Heterocyclic compound and salt thereof, and luminescent substrate composition

An object of the present invention to provide a firefly luciferin and firefly luciferin analog that are modified to maintain luminescent activity by luciferase in a firefly bioluminescent system. In particular, it is an object of the present invention to provide a new luminescent substrate for which the emission wavelength in a firefly bioluminescent system is shifted to a longer wavelength than that of a conventional luminescent substrate. The present invention provides a luciferin in which the benzothiazole ring moiety has been modified at the 7-position, a luciferin analog in which the benzene ring moiety has been modified at the 6-position, and a luciferin analog in which the 6-(dialkylamino)-2-naphthalenyl moiety has been modified at the 5-position.

Luminescent substrate for luciferase

The present invention relates to a compound having a structure analogous to firefly luciferin. In particular, the invention relates to a heterocycle compound which produces a luminescence at a light wavelength different from that of firefly luciferin in nature. The present invention provides a heterocycle compound of following general formula I. In the above general formula, R, Rand Rcan be each independently H or C-alkyl. In the above general formula, X and Y can be each independently C, N, S or O. In the above general formula, the olefin chain part expressed as 'n' can be changed to desired length. 

Luminescent substrate for liciferase

Growing community of inventors

Tokyo, Japan

Shojiro Maki