The University of Tokyo

Dai Nippon Printing Co., Ltd.

Japan Science and Technology Agency

Texas Instruments Corporation

Ntt Docomo, Inc.

Terumo Kabushiki Kaisha

Fujikura Limited

Toyota Boshoku Kabushiki Kaisha

Nippi Corporation

Kanagawa Institute of Industrial Science and Technology

Mitsubishi Chemical Medience Corporation

Nissin Foods Holdings Co., Ltd.

Lsi Medience Corporation

Shoji Takeuchi

Hiroaki Onoe

Hiroaki Suzuki

Ryoichi Ohigashi

Daisuke Kiriya

Yukiko Matsunaga

Koji Fujimoto

Yutaka Ozawa

Teru Okitsu

Keisuke Tanaka

Hiroyuki Fujita

Satoshi Yamamoto

Sadao Ota

Teruo Fujii

Satoshi Hiyama

Osamu Hayashida

Hiroyuki Noji

Yasuyuki Sakai

Tetsuro Kawanishi

Masakazu Sugiyama

Nobuhito Mori

Shunji Hattori

Hideaki Shibata

Hitoshi Matsui

Itaru Hamachi

Osamu Nukaga

Toshihisa Osaki

Riho Gojo

Tetsuya Yamada

Hisatoshi Mimura

Hirotaka Sugiura

Kanae Sakai

Yuya Morimoto

Shigenori Miura

Wataru Narishima

Naoko Teramura

Masato Ikeda

Ryuji Yokokawa

Katsumasa Iijima

Kazuhito Tabata

Kaori Kuribayashi

Yukiko Tsuda

Kei Funakoshi

Wei-Heong Tan

Midori Negishi

Yun-Jung Heo

Ai Shima

Provided is a culture method for a microorganism using a plate-like microchamber having a plurality of wells independent from each other on a front surface thereof. The culture method for the microorganism includes: mixing a liquid culture medium containing agarose having a gelation temperature of no less than 5° C. and no more than 20° C. and the microorganism to obtain a mixture; enclosing the mixture into the well with the front surface facing upward; and keeping the microchamber in which the mixture is enclosed in the well at a predetermined temperature range.

Culture method

The present invention provides a measuring device which includes: a first container and a second container that are arranged adjacent to each other; and a partition wall provided between the first and second containers, the partition wall having a through hole(s) for forming a lipid bilayer(s). In this measuring device, a gas flow path(s) having a hydrophobic surface is/are formed in at least one of the first and second containers; the gas flow path(s) has/have an inlet and an outlet; the inlet and the outlet each communicate with the exterior of the measuring device; the gas flow path(s) open(s) into the container in which it/they is/are formed; and a gas flowing in the gas flow path(s) contacts the droplet filled in the container.

Measurement instrument and method for measuring target substance using same

The method for producing a three-dimensional artificial tissue is a method in which a three-dimensional artificial tissue extending in a predetermined direction is produced. The method includes: preparing a device including a culture tank having a culturing space surrounded by sidewalls, and a flow channel-forming member that penetrates through the sidewalls that face each other and is suspended in the culturing space along a predetermined direction; culturing cells in the culturing space and thereby forming a three-dimensional artificial tissue through which the flow channel-forming member penetrates; and removing the flow channel-forming member from the three-dimensional artificial tissue and thereby forming a perfusion flow channel that penetrates through the three-dimensional artificial tissue.

Three-dimensional artificial tissue, method for producing the same, three-dimensional artificial tissue perfusion device, and drug evaluation method using three-dimensional artificial tissue

The present invention relates to a hollow microfiber comprising (1) one or more cell-adhesive layers having a cell-adhesive hydrogel, (2) an outer shell layer having a high-strength hydrogel that covers the outer periphery of the cell-adhesive layer that is positioned farthest from the center axis among the one or more cell-adhesive layers, and (3) a cell layer that covers the inner periphery of the cell-adhesive layer that is positioned closest to the center axis among the one or more cell-adhesive layers. The present invention also relates to a method of manufacturing the hollow microfiber and a kit for carrying out the manufacturing method.

Hollow microfiber

Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from-derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which-derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

[object Object]

A method for preparing a linearly extended supramolecular fiber or a plurality of linearly aligned supramolecular fibers, which comprises the step of allowing supramolecular monomers to be self-assembled in a microfluidic channel.

Process for producing supramolecular fiber

Disclosed is a substrate (A) for trapping a microorganism or cell (T), characterized by comprising a base () and having a space () into which a fluid (R) containing the microorganism or cell (T) is introduced and a microfine suction hole () through which the space () communicates with the outside of the base (). The substrate is further characterized in that the space () has been formed in the base (), and at least the portion of the base () which forms the microfine suction hole () is constituted of a single member.

Base body and method of manufacturing base body

A method for fabricating a microarray of plural soft materials includes: vapor-depositing a first layer poly(para-xylylene) resin on a substrate, forming a first micro pattern in the poly(para-xylylene) resin; obtaining a substrate including a first microarray formed by pouring a first soft material solution, freeze-drying the first soft material to obtain a micro-arrayed substrate of the freeze-dried first soft material; vapor-depositing a second layer poly(para-xylylene) resin on the micro-arrayed substrate of the freeze-dried first soft material, forming a second micro pattern placed differently from the first micro pattern by penetrating the poly(para-xylylene) resin of the first and second layers, forming a second microarray on the substrate by pouring a second soft material solution; and forming a microarray of the first and second soft materials on the substrate by peeling off the poly(para-xylylene) resin of the first and second layers.

Method for fabricating a microarray of soft materials

A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.

Method for culture of hepatocytes

To provide a fluorescent hydrogel having superior detectability of saccharides such as glucose and minimal invasiveness, a method for producing the same, and a sensor for measuring saccharides using the same. A florescent hydrogel having a structure represented by the following chemical formula 1, a method for producing the same, and a sensor for measuring saccharides using the same. 

Fluorescent hydrogel and method for producing the same, and sensor for measuring saccharides using the same

A microfiber showing improved mechanical strength, which comprises a micro gel fiber consisting of collagen gel or the like covered with high strength hydrogel such as alginate gel.

Covered micro gel fiber

There is provided a planar lipid bilayer array formed by microfluidic technique and a method of analysis using the planar lipid bilayers, providing the advantages such as portability, decreased analysis time, a smaller amount of required reagents, and parallel automation with high reproducibility. The planar lipid bilayer array formed by microfluidic technique is a planar lipid bilayer array formed by microfluidic technique (PDMS device)saturated with water by preliminarily immersing in water, comprising microchannelsconnected to an inlet of a microfluidic channel and arranged in parallel, and microchambershaving apertures on both sides of the microchannel

Planar lipid bilayer array formed by microfluidic technique and method of analysis using planar lipid bilayer

The present invention provides simple and an accurate apparatuses for forming bilayer membranes by the contact of amphipathic monolayers. In one apparatus, amphipathic monolayers are brought into contact and fused by controlling the pressure of liquids introduced from microchannels forming an intersection containing a chamber. In another apparatus, the chamber includes a number of compartments and at least one construction portion. In this apparatus, a droplet is formed within each compartment and contact of amphipatic monolayers is accomplished by adjusting the size of the droplets.

Method of forming bilayer membrane by contact between amphipathic monolayers and apparatus therefor

A microchip for forming an emulsion has a first glass substrate, a second glass substrate and a silicon substrate. The silicon substrate has formed therein a first fluid flow path through which a first fluid flows and a second fluid flow path through which a second fluid that is not mixed with the first fluid flows. The first fluid flow path has a plurality of branched flow paths that join at a joint portion. The second fluid flow path communicates with the joint portion. The silicon substrate has formed therein an emulsion formation flow path that faces an edge portion of the second fluid flow path at the joint portion. An emulsion composed of the first fluid and the second fluid that is surrounded by the first fluid is formed in the emulsion formation flow path.

Microchip for forming emulsion and method for manufacturing the same

Microchip for forming emulsion

Growing community of inventors

Tokyo, Japan

Shoji Takeuchi