Mitsui Toatsu Chemicals, Incorporated

Shoichiro Miyahara

Kazunari Nitta

Toshio Matsumoto

Nobuyoshi Makiguchi

Tooru Miyahara

Maki Suzuki

Nobumi Kusuhara

Atsunori Shindo

Akio Sakaguchi

Takeshi Nakamura

Ken-ichi Ishiwata

A method for the production of human tissue type plasminogen activator (human t-PA) using cells is disclosed. The method comprises producing the human t-PA in parallel with the growth of the cells or after the cells have been grown. To the cell culture bicarbonate ion is added at a concentration equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.

Method for production of human tissue type plasminogen activator

Optically active serine can be racemized at a high reaction rate under the relatively mild conditions by treating the optically active serine in an alkaline solution in the presence of pyridoxal phosphate or salicylaldehyde and an alkaline compound.

Method for racemization of optically active serine

A process for purifying tryptophan so as to contain almost no unreacted indole and a process for recovering unreacted indole with a very high percentage are provided, the purification process comprising passing a reaction fluid of tryptophan prepared from indole as raw material by means of an enzyme function, through a porous type, cation exchange resin layer, to cause the ion exchange part of the resin to absorb tryptophan, while to cause the porous part of the resin to adsorb indole, followed by eluting only the tryptophan with an alkali or acid aqueous solution, and the recovery process comprising subjecting tryptophan to repeated treatments of adsorption onto and elution from the ion exchange part of the resin and regeneration followed by eluting indole adsorbed onto the porous part of the resin with a water-containing organic solvent miscible with water and isolating indole from the resulting elute.

Process for purifying tryptophane

Disclosed is a method for separating lycine and L-serine from a solution containing both of them in the dissolved state and, in particular, a reaction solution obtained from a fermentation or enzymic conversion process for the preparation of L-serine by using glycine as the raw material. This method is based on a chromatographic technique utilizing the differential affinity of L-serine and glycine for a strongly acidic ion exchange resin.

Method for separating glycine and L-serine from a solution containing

Growing community of inventors

Yokohama, Japan

Shoichiro Miyahara