Mitsui Toatsu Chemicals, Incorporated

Shizuo Shimada

Yoshio Furutani

Hiroshi Yamamoto

Osamu Yano

Daiji Iwata

Tamotsu Fukuda

Wakao Sato

Tadashi Sudo

Hitoshi Inoue

Yoshikazu Fujisawa

Disclosed is a novel anti-ganglioside GD.sub.1a monoclonal antibody (MZ) which is capable of recognizing ganglioside GD.sub.1a but is practically incapable of recognizing GalCer, LacCer, Gb.sub.3, Gb.sub.4, GA.sub.1, GA.sub.2, GM.sub.2, GM.sub.3, GD.sub.1b, GT.sub.1b, GQ.sub.1b, Fuc-GM.sub.1, nLc.sub.4 and sialosyl nLc.sub.4 ; MZ-producing cells; an MZ-containing reagent; and a method for the detection or quantification of GD.sub.1a using the reagent, e.g., to diagnose cancer, systematic lupus erythematosus and diseases resulting from organic injury of the nervous system.

Anti-ganglioside GD.sub.1 A monoclonal antibody MZ, MZ-producing cells

Cells of a microorganism, such as Mycobacterium tuberculosis, belonging to the genus Mycobacterium are disrupted in distilled water or a suitable buffer solution, and then centrifuged or filtrated to remove the cell wall residue. To the aqueous cell-free extract is added a polyvalent metal salt or an antibiotic, such as streptomycin sulfate, which acts as a flocculant to form a precipitate. The precipitate consists essentially of sugar, protein, lipid, and nucleic acid. Owing to its high antitumor and adjuvant activities as well as its slight side effects, it can be used as antitumor and adjuvant preparations.

Bacterial cell extract, process for preparing same, antitumor

Thermally-denatured DNA MD-011 having an antitumor activity was obtained by heating the substance separated from the disrupted cells of bacteria belonging to genus Mycobacterium.

Thermally-denatured deoxyribonucleic acid MD-011 and antitumor agent

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Mobara, Japan

Shizuo Shimada