Hitachi, Ltd.

Hitachi-high-technologies Corporation

The University of Tokyo

Kyoto University

Kobe University

Shizu Takeda

Masaharu Kiyama

Maiko Tanabe

Guangbin Zhou

Ryota Nakajima

Hiroko Hanzawa

Masakazu Sugaya

Koichi Terada

Taku Nakamura

Takayuki Nozaki

Kunio Ohyama

Daisuke Suzuki

Kazumichi Imai

Hiroaki Nakagawa

Yoko Inoue

Masahiro Okanojo

Midori Kato

Kiyoto Ito

Akihiro Shimase

Naoko Senda

Hikaru Saito

Masao Washizu

Yumiko Igarashi

Kennedy Omondi Okeyo

Yuki Niimi

Osamu Imaichi

Michihiro Araki

Kenji Tsuge

A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.

Functional sequence selection method and functional sequence selection system

A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.

Cell culture method and automatic cell culture apparatus

The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.

Cell culture substrate, cell culture vessel, and method for cell culture

A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.

Liquid delivery device and cell culture device using the same

There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipeis connected with a gas bag, the liquid to be fed into the feed pipeis fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a containerfor holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container.

Liquid feeding device and cell culture device

In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vesselholding a biological sample therein, a heat storage portionholding the culture vessel, and a heat insulation portionsurrounding the heat storage portion. The whole circumference or a part of, andof the heat insulation portionis removable depending on an arrangement environment.

Cell culturing device, culturing vessel, and holding vessel

There is provided a method for noninvasively evaluating the cell state (proliferation, multi-layering, and differentiation) of a cell sheet as a mimic tissue at the time of culturing the cell sheet. The method is characterized in that an analysis of an amino acid is conducted with the use of the culture supernatant of a cell sheet to monitor a change in the concentration of any amino acid selected from a group of 5-species of amino acids (Ile, Val, Ser, Leu, and Ala), thereby making a determination.

Method for determining cell state and autoanalyzer using said method

Growing community of inventors

Tokyo, Japan

Shizu Takeda