Nanjing University

Shixiang Yang

Xiaolong Wang

Wenjin Hu

Xiang Long

Huai Lin

Yi Luo

Shengyang Wang

Disclosed are a nanobody targeting influenza A virus nucleoprotein and an application thereof, and a nucleic acid encoding the nanobody. The present disclosure obtains one nanobody targeting influenza A virus nucleoprotein through construction of a nanobody phage library and phage screening technology. The nanobody targeting influenza A virus nucleoprotein is a VHH antibody fragment having an amino acid sequence shown in SEQ ID NO:1-SEQ ID NO:2. The nanobody in the present disclosure can specifically bind to influenza A virus nucleoprotein, and can be applied to the detection and analysis of influenza A virus, and has broad application prospects in the fields of early warning of the epidemic or outbreak of influenza A virus, as well as the detection of influenza A virus in the environment or human body.

Nanobody targeting influenza a virus nucleoprotein and application thereof, and nucleic acid encoding nanobody

Disclosed are a preparation method and application of a single-chain antibody fragment targeting SARS-CoV-2 nucleocapsid protein, aiming to provide a single-chain antibody fragment with high affinity and strong specificity. The present disclosure obtains three single-chain antibody fragments targeting SARS-CoV-2 nucleocapsid protein through construction of a nanobody phage library and phage screening technology. The single-chain antibody fragment consists of a heavy chain variable region, a 15aa connecting peptide, and a light chain variable region connected in sequence, and has an amino acid sequence shown in SEQ ID NO: 1-SEQ ID NO: 3. The single-chain antibody fragment in the present disclosure has excellent binding performance and specificity with the SARS-CoV-2 nucleocapsid protein, can be applied to a variety of immunoassay platforms of the SARS-CoV-2, and has broad application prospects in the field of SARS-CoV-2 detection.

Preparation method and application of single-chain antibody fragment targeting SARS-CoV-2 nucleocapsid protein

Disclosed are a preparation method and application of a nanobody targeting dengue virus NS1 protein. The nanobody targeting dengue virus NS1 protein is a VHH antibody, which is obtained by constructing a nanobody phage library through phage screening technology, and has an amino acid sequence shown in SEQ ID NO: 1. The nanobody in the present disclosure exhibits high affinity and specificity when being bound to the dengue virus NS1 protein, and has advantages of good stability and low cost compared with monoclonal antibodies. Therefore, the nanobody in the present disclosure can be produced on a large scale, and has bright application prospects in the field of dengue virus detection and analysis.

Growing community of inventors

Jiangsu, China

Shixiang Yang