Jiangnan University

Shixia Xi

Zhengbiao Gu

Zhaofeng Li

Caiming Li

Xiaofeng Ban

Zexi Li

Xiao Zhang

The invention provides an oligosaccharide debranching enzyme mutant and use thereof in a glucose mother liquor. The mutant is obtained by mutating valine at position 219 in SEQ ID NO: 1 into alanine. According to oligosaccharide debranching enzyme mutant V219A, a primary mother liquor, a secondary mother liquor, or a tail liquid after chromatographic separation is used as a substrate, the percentage contents of glucose in the products are 99.21% (primary mother liquor), 98.89% (secondary mother liquor) and 97.97% (tail liquid after chromatographic separation) respectively, which are 2.86%, 8.64%, and 28.67% higher than that of glucose obtained with the wild-type oligosaccharide debranching enzyme. Therefore, the mutant V219A obviously improves the percentage content of glucose in the glucose mother liquor, and the scope of application of the mother liquor can be expanded by the high product purity and substrate conversion rate, so the mutant V219A has higher industrial application value.

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Wuxi, China

Shixia Xi