Council of Scientific and Industrial Research

Shiv Kumar Gupta

Sushil Kumar

Rakesh Tuli

Madan Mohan Gupta

Dharam Chand Jain

Suman Preet Singh Khanuja

Atul Prakash Kahol

Ram Kishor Verma

Samir Vishwanath Sawant

Pradhyumna Kumar Singh

Savithri Bhat

Ajit Kumar Shasany

Mahendra Pandurang Darokar

Rajendra Singh Bhakuni

Sudeep Tandon

Digvijay Singh

Amit Tewari

Alok Kumar Srivastava

Govind Ram

Neerja Pant

The invention relates to a chemically synthesized artificial promoter comprising a DNA sequence designed for the target level and pattern of gene expression, by strategically putting together several signature sequences identified by sequence alignment and statistical analysis of a large database constructed for this purpose.

Chemically synthesized artificial promoter for high level expression of transgenes and a method for its synthesis

The present invention relates to a process for the isolation of artemisinin, an antimalarial agent from the herb of the plant, comprising of extracting the herb with ethanol, partitioning of the extract between water and hexane, followed by evaporative crystallization of artemisinin from hexane phase to produce substantially pure artemisinin.

Process for isolating artemisinin from Artemisia annua

Chemically synthesized artificial promoter for high level expression of transgenes

The invention provides a method for maximization of artemisinin yield of the plant , said method comprising sowing seeds of plant on raised bed nursery during second and third week of December and maintaining the moisture throughout; transplanting seedlings thus obtained bearing at least 5-15 leaves into the main field fertilized with fertilizer, preferably NPK &commat; 80,40,40 kg/ha to achieve a population density of 50,000 to 200,000 per ha followed by light irrigation in the second week of March and irrigation every fortnight thereafter; harvesting the crop four times by cutting the plant tops leaving 75-100 cm part of plant for further regeneration, the said harvests are performed in a manner that the first harvest is done in fourth week of May, second harvest in third week of July, third harvest in second week of September and fourth harvest in third week of October of each year; and at each harvesting time care is taken to care at least one green branch, and extracting artemisinin from the plant tissue immediately after each harvest.

Method for maximization of artemisinin production by the plant artemisia annua

The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.

Process for the isolation of compound scopoletin useful as nitric oxide synthesis inhibitor

The present invention relates to a tissue culture process for producing a large number of genetically transformed viable mint plants in vitro. The process of the present invention employs specified pieces of an internodal segment of the stem of the mint plant as the starting material and identifies medium, culture and transformation conditions for producing a large number of genetically transformed plants. Such plants can be selected for desirable characteristics.

Tissue culture process for producing a large number of viable mint plants in vitro

The present invention relates to a tissue culture process for producing a large number of viable cotton plants in vitro from a specified tissue of cotton plant. The invention provides genotype independent, direct, multiple shoot proliferation and opens up new possibilities for micropropagation, selection of mutants and for producing genetically improved cotton plants by modern methods of agrobiotechnology and genetic engineering. The protocol provides an important step in the success of cotton improvement programmed, utilizing tissue culture technology.

Tissue culture process for producing a large number of viable cotton plants in vitro

The present invention relates to a tissue culture process for producing a large number of viable mint plants in vitro. The process of the present invention employs specified pieces of an internodal segment of the stem of the mint plant as the starting material and identifies medium and culture conditions for producing a large number of plants. Such plants can be used for micropropagation, selection of mutants, production of plants with altered levels of endogenous secondary metabolites and for genetic engineering.

Tissue culture process for producing a large number of viable mint

Growing community of inventors

Lucknow, India

Shiv Kumar Gupta