Qiagen Gaithersburg Inc.

Qiagen Gaithresburg, Inc

Shiuli Agarwal

Irina A Nazarenko

Arvind Virmani

Dominic O'Neil

Holly Basham

Thomas Rothmann

Brian Lowe

Mridula Shukla

Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step using two separate sequence-specific polynucleotide probes. Also provided are nucleic acids comprising SEQ ID NO: 1 to SEQ ID NO: 727 and nucleic acid probes and probe sets comprising the same.

Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step comprising contacting the target nucleic acid with a plurality of detectably labeled nucleic acid detection probes, wherein each (a) bears a different detectable label from the other detection probes, and/or (b) has a different melting temperature from probes bearing the same detectable label. Also disclosed are compositions and kits for use in such a method.

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Somerville, MA, United States of America

Shiuli Agarwal