Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Universiteit Stellenbosch

Mascoma Corporation

Shital A Tripathi

Aaron Argyros

Nicky Caiazza

John E McBride

Allan C Froehlich

Elena E Brevnova

Trisha Barrett

Charles F Rice

Arthur J Shaw, Iv

Erin Wiswall

Anne K Warner

Mark Mellon

Ryan Skinner

Riaan Den Haan

Emily Stonehouse

Chhayal V Gandhi

Haowen Xu

Kristen M Deleault

William Ryan Sillers

John S Bardsley

Kevin S Wenger

Indraneel Shikhare

Frank Agbogbo

Jennifer Gosselin

Yin-Ying Chiu

Vineet B Rajgarhia

Alan Belcher

Abigail Foster

Heidi H Hau

David Anthony Hogsett

Vineet Rajgarhia

Johannes Pieter Van Dijken

James H Flatt

Heidi Lau

Elena Brevnova

John E Mcbride

Abigail S Foster

Arthur J Iv Shaw

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.

Production of malonyl-COA derived products via anaerobic pathways

The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms

Production of malonyl-CoA derived products via anaerobic pathways

Growing community of inventors

Berkeley, CA, United States of America

Shital A Tripathi