Nara Institute of Science and Technology

Shinya Takenami

Ko Kato

Shigehiko Kanaya

Hideyuki Matsuura

Tatsuya Nosho

Yuhki Kubo

Kiyotaka Ueda

An object of the present invention is to identify sequence features of the 5' UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5&#x2032; UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector: (a) 5&#x2032; UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5&#x2032; end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and (b) 5&#x2032; UTR in which one or more bases in the base sequence of the 5&#x2032; UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.

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Yokohama, Japan

Shinya Takenami