Nec Solution Innovators, Ltd.

Kanagawa Prefectural Hospital Organization

Ohtsu, Takashi

Tsuji, Shotaro

Tsuji, Makiko

Shintarou Katou

Iwao Waga

Jou Akitomi

Katsunori Horii

Shotaro Tsuji

Takashi Ohtsu

Naoto Kaneko

Hiromi Takenaka

Yoshihito Yoshida

Makio Furuichi

Mineko Yamaguchi

Hirotaka Yagi

Kensaku Nishikata

Naomi Hirabayashi

Ohtsu Takashi

Tsuji Makiko

Tsuji Shotaro

The present invention provides a novel technique by which the redox activity of a nucleic acid molecule can be evaluated. An evaluation method of the present invention includes: a detection step of electrochemically detecting a redox reaction to a substrate, the redox reaction being catalyzed by a nucleic acid molecule to be evaluated, using a device that electrochemically detects a redox reaction; and an evaluation step of evaluating redox activity of the nucleic acid molecule from a result of the detection of the redox reaction. As the device, a device in which a base provided with a detection portion is included, the detection portion includes an electrode system, and the nucleic acid molecule to be evaluated is arranged on the base is used. In the present invention, it is preferred that a plurality of kinds of nucleic acid molecule to be evaluated is arranged on the base, and the plurality of kinds of nucleic acid molecules to be evaluated is evaluated by a single device.

Method for evaluating redox activity of nucleic acid molecule and nucleic acid molecule having redox activity

The present invention provides a novel sensor for detecting a target. The nucleic acid sensor of the present invention includes a nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to a target. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (S), a loop-forming sequence (L2), and a stem-forming sequence (S) linked in this order. In this nucleic acid element, in the absence of a target, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (S) and (S), and in the presence of ATP/target, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the target, and the catalytic function is exerted.

Device and method for analyzing target

The present invention is to provide a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the present invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 &#x3bc;M or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the present invention includes the binder for a rodent-derived IgG antibody of the present invention. The detection kit for detecting a rodent-derived IgG antibody of the present invention includes the detection reagent for detecting a rodent-derived IgG antibody of the present invention.

Nucleic acid molecule having binding affinity to rodent-derived IgG antibody, binder, detection reagent, and detection kit

The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present inventionincludes an input unit, a calculation unit, and a prediction unit. The input unitis a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unitcalculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unitcompares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.

Prediction device, prediction method, program, and recording medium

An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide may be a nucleic acid containing the base sequence of SEQ ID NO: 17, SEQ ID NO: 18, or containing a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in SEQ ID NO: 17 or 18.

Aptamer that recognizes peptide

A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5&#xd7;10or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.

HMGB1 binding nucleic acid molecule and applications thereof

The invention provides a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 &#x3bc;M or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the invention includes the binder for a rodent-derived IgG antibody of the invention. The detection kit for detecting a rodent-derived IgG antibody of the invention includes the detection reagent for detecting a rodent-derived IgG antibody of the invention.

The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2).

Growing community of inventors

Tokyo, Japan

Shintarou Katou