Denka Company Limited

Shino Muramatsu

Daisuke Kato

Tomohiro Hattori

This invention provides a method and an immunochromatographic device that enable measurement with sufficient sensitivity by performing nitrous acid extraction over a sufficiently long period of time in an immunochromatographic method comprising extracting a sugar chain antigen with nitrous acid and measuring the extracted sugar chain antigen on an immunochromatographic test strip. Such immunochromatographic device comprises a specimen dropping port on top of a sample pad of the test strip. The immunochromatographic device has: (i) a wide specimen dropping port to promote sugar chain antigen extraction with a nitrite and a solid acid reagent by retaining the added specimen sample solution and supplying the specimen sample solution to the region impregnated with the solid acid reagent or the nitrite within a short period of time; and (ii) no gaps between the dropping port and the sample pad so as to prevent the sample from leaking from the dropping port.

Immunochromatographic device for extracting and measuring sugar chain antigen

The present invention relates to an immunochromatography test method of measuring a sugar chain antigen, which provides an immunochromatographic test piece and a specimen adding device capable of specifically measuring a sugar chain antigen, and an immunochromatography method using the same; wherein after mixing a specimen with a nitrite solution, a step of allowing tartaric acid to come into contact with the mixture, and extracting a sugar chain antigen contained in the specimen is carried out in a filtration step.

Immunochromatographic test piece and specimen adding device for extracting and measuring sugar chain antigen, and immunochromatography method using same

The present disclosure provides an immunochromatographic test piece which prevents a non-specific reaction by efficiently and continuously contacting and neutralizing a developing solution containing nitrous acid with a neutralizing reagent in an immunochromatography method of extracting and measuring a sugar chain antigen by nitrous acid extraction on the immunochromatographic test piece. The present disclosure also provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, which comprises: a sample pad; a label region; a detection region on which the antibody against the sugar chain antigen is immobilized; a region impregnated with a neutralizing reagent upstream of the label region, and a region impregnated with a solid acid reagent or a region impregnated with nitrite, upstream of the region impregnated with the neutralizing reagent.

Immunochromatographic test piece for extracting and measuring sugar chain antigen, which is capable of preventing non-specific reaction

It is an object of the present invention to provide a method or an immunochromatographic test piece, which controls a speed or a direction of development of a specimen on the immunochromatographic test piece, so that a treatment with an acid reagent, nitrite, and a neutralizing reagent is properly controlled. The present invention provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, the immunochromatographic test piece having a neutralizing region impregnated with a neutralizing reagent upstream of a label region, and further having a solid acid reagent or nitrite region impregnated with a solid acid reagent, or with nitrite, upstream of the neutralizing region, wherein a resin-made sheet is sandwiched between the solid acid reagent or nitrite region the neutralizing region so as to suppress the movement of a reagent or the movement of a specimen solution between the regions.

Immunochromatographic test piece for extracting and measuring sugar chain antigen, which is capable of controlling development of specimen

Methods of agglutinating and separating erythrocytes, by which erythrocytes can be instantaneously agglutinated into a sufficient size in a blood sample and completely separated from the blood sample; and a hemagglutination reagent are provided. The method of agglutinating erythrocytes according to the present invention includes adding a solution containing a cholic acid-based surfactant and an acid to a blood sample. The method of separating erythrocytes according to the present invention includes separating the erythrocytes agglutinated by the above-described method of the present invention. The hemagglutination reagent according to the present invention contains a cholic acid-based surfactant and an acid.

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Niigata, Japan

Shino Muramatsu