Kabushiki Kaisha Toshiba

Shinji Yanaga

Sadato Hongo

Jun Okada

Minoru Ishikawa

Shigeru Wakayama

Kenji Oki

Takiji Ishimura

A method for determining nucleotide sequence encompasses: injecting a solution containing a sample DNA into a chip cartridge provided with a detecting electrode, to which a probe DNA is immobilized; introducing an intercalator solution in the chip cartridge; obtaining a current-voltage characteristic curve by measuring a current in the solution due to an electrochemical reaction of the intercalator through the detecting electrode; obtaining a baseline by linearly approximating the current-voltage characteristic curve; obtaining a net current value by subtracting, from a peak current value of the current-voltage characteristic curve, a baseline current value obtained from the baseline at a peak voltage value defining the peak current value; and identifying a nucleotide sequence in the sample DNA, using the net current value.

Method and an apparatus for determining nucleotide sequence, and a computer program product to be executed by the apparatus

A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.

Base sequence detection apparatus and base sequence automatic analyzing apparatus

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Yokohama, Japan

Shinji Yanaga