Japan Tobacco Inc.

Shinji Morioka

Jun Ueki

Shozo Ohta

Yoshiki Kuraya

A novel nucleic acid fragment which has an high activity to promote expression of a structural gene located downstream thereof, and a method for promoting expression of the structural gene located downstream thereof using the nucleic acid are disclosed. A nucleic acid fragment having the nucleotide sequence shown in SEQ ID NO:1 in the Sequence Listing, and nucleic acid fragments having the same sequence as shown in SEQ ID NO:1 except that one or more nucleotides are substituted or deleted, or one or more nucleotides are inserted therein or added thereto, which have activities to promote expression of a structural gene located downstream thereof were provided.

Nucleic acid fragment, recombinant vector containing the same and method of promoting the expression of structural genes by using the same

A method for expressing a foreign gene, by which the foreign genes can be expressed more strongly than by the conventional methods, as well as a recombinant vector therefor, is disclosed. In the method for expressing a foreign gene according to the present invention, the foreign gene is inserted into a site downstream of a promoter and the foreign gene is expressed in a cell, wherein a plurality of intron-originated DNA fragments which are the same or different and are capable of promoting expression of foreign genes are inserted into one or more sites upstream of the foreign gene, and said foreign gene is expressed.

Method for the expressing foreign genes and vectors therefor

Method of changing the composition of the phospholipid produced by the

DNA fragment, recombinant vector containing the same and method for

Phospholipase D gene originated from plant

Growing community of inventors

Shizuoka, Japan

Shinji Morioka