Meiji Seika Kaisha, Ltd.

Shinji Miyado

Yujiro Yamada

Taro Niida

Takashi Shomura

Tomizo Niwa

Chuhei Nojiri

Hitoshi Goi

Hiroshi Watanabe

Akira Suzuki

Takashi Tsuruoka

Shigeharu Inouye

Shigeo Seki

Fumio Kai

Norio Ezaki

Shoji Omoto

Shoichi Amano

Mitsugu Ito

L-2-Amino-4-methylphosphinobutyric acid (L-MPGA) which is useful as bactericide and herbicide can be prepared by optical resolution of its racemic mixture, D,L-MPGA with the action of a microbial acylase. The process comprises providing D,L-MPGA in the form of its N-acyl derivative, reacting N-acyl-D,L-MPGA in an aqueous medium with a microbial acylase having an optically specific hydrolytic activity, whereby selectively eliminating the acyl group of N-acyl-L-MPGA to give a mixture of L-MPGA and N-acyl-D-MPGA and isolating L-MPGA from the mixture. The acylase is produced by cultivating a microbial strain belonging to the genus Pseudomonas, Streptomyces or Aspergillus, particularly Pseudomonas maltophilia BN-233, Streptomyces violascens SF 2072, Streptomyces diastatochromogenes SF 2073, Aspergillus sp. KS-101 or Aspergillus sp. KS-102.

Process for the optical resolution of

Compounds are provided having the general formula I: ##STR1## wherein X represents an hydroxyl group, an acetoxy group or a thiosulfuric acid residue. The formula I compounds are prepared from compounds of the general formula II: ##STR2## wherein R.sub.1 represents hydrogen, a lower alkanoyl group, an arylalkanoyl group, an alkoxycarbonyl group, a lower haloalkoxycarbonyl group, a substituted or unsubstituted aroyl group, a N-arylcarbamoyl group of a substituted or unsubstituted aryl group; R.sub.2 either represents hydrogen or, together with the ##STR3## group, represents a phthalimido group; and X represents an hydroxyl group, an acetoxy group or a thiosulfuric acid residue, or their metal salts or their salts with organic bases, by causing the culture of a cephalosporin C acylase-producing strain of a microorganism, especially a mold, fungus, or the secondary preparation therefrom, to act on the latter compounds II in the presence of an aqueous medium.

Process for the preparation of 7-amino-cephem compounds using mold fungi

A new antibiotic designated BN-130 substance is now provided, which has antibacterial activity against gram-negative and gram-positive bacteria as well as acid-fast bacteria. BN-130 substance is produced by cultivating a strain of Pseudomanas stutzeri under aerobic conditions.

Antibiotic BN-130 substances and the production thereof

A BN-109 substance producing strain of the genus Bacillus is cultivated under an aerobic condition to produce and accumulate BN-109 substance in a culture medium. The BN-109 substance is isolated from the culture broth and purified, and proved to be a novel antibiotic. The BN-109 is effective in the treatment and prevention of swine scours.

Novel antibiotic BN-109 substance, its production and use

Growing community of inventors

Yokohama, Japan

Shinji Miyado