Sumitomo Chemical Company, Limited

Shinji Hourai

Yasushi Matsuki

Norihiko Hirata

Toshihiko Akiyama

The present invention relates to, for example, an &#x3b1;-substituted &#x3b2;-amino acid ester derivative asymmetric hydrolase including an enzyme of the following (a) or (b): (a) an enzyme comprising the amino acid sequence of SEQ ID NO:1 at least from position 1 to position 362, wherein the tyrosine at position 277 of SEQ ID NO:1 is substituted with alanine, tryptophan, isoleucine, or histidine, and having the ability to hydrolyze a substrate; or(b) an enzyme comprising the amino acid sequence of SEQ ID NO:1 at least from position 1 to position 362, wherein the tyrosine at position 277 of SEQ ID NO:1 is substituted with an amino acid other than tyrosine, and having the ability to hydrolyze a substrate.

Asymmetric hydrolase and gene thereof

The present invention relates to an isolated or modified esterase having the excellent thermostable property which can be utilized for ester hydrolysis reaction, ester synthesis reaction, ester interchange reaction and its gene. The esterase is characterized in that it has at least a partial amino acid sequence necessary for expressing the thermostable esterase activity among the amino acid sequence shown by SEQ ID NO: 2 and further has an amino acid substitution described herein.

Growing community of inventors

Toyonaka, Japan

Shinji Hourai