Tokyo Medical University

Bonac Corporation

Shinichiro Ohno

Masahiko Kuroda

Tadaaki Ohgi

Eriko Aoki

Shiori Kato

Yasuhiko Yoshida

The invention provides an artificial match-type miRNA utilizing miRNA. In particular, the invention provides a single strand nucleic acid containing an X region and a Y region, wherein the 3'-terminal of the X region and the 5&#x2032;-terminal of the Y region are linked via a linker region of a non-nucleotide structure, the X region contains a guide strand sequence of a mature miRNA, and the Y region contains a sequence completely complementary to the X region is an artificial match-type miRNA. The artificial match-type miRNA can suppress expression of the target gene.

Artificial match-type miRNA for controlling gene expression and use therefor

The present disclosure is directed to an artificial mimic miRNA utilizing miRNA. An artificial mimic miRNA is a single-stranded nucleic acid including: a X region; and a Y region, the Y region and the X region being linked, wherein the X region is a guide strand sequence of a mature miRNA or a partial sequence of the guide strand sequence of the mature miRNA and consists of a linking side region (X) and a non-linking side region (X) to the Y region, the linking side region (X) is a sequence that does not cause intramolecular annealing within its region, and the Y region is a sequence that intramolecularly anneals to the non-linking side region (X) of the X region. According to the artificial mimic miRNA of the present invention, the expression of the target gene can be inhibited.

Artificial mimic miRNA for controlling gene expression, and use of same

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Tokyo, Japan

Shinichiro Ohno