Sysmex Corporation

Shinichiro Oguni

Tomohiro Tsuji

Ayumu Yoshida

Daigo Fukuma

Masanori Imazu

Yoshiro Ikeuchi

Masaki Abe

Yukiko Kataoka

Takaaki Nagai

Toshihiro Mizukami

Tatsuya Narikawa

Saori Suzuki

Jo Lissen

Yuji Itose

Jo Linssen

Seido Biwa

Keiko Moriyama

Saori Otsuka

Kazuki Hatcho

The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The present invention also provides a regent kit and reagent for classifying leukocyte which are used for classifying and counting leukocytes in biological samples.

Method for classifying/counting leukocytes, reagent kit for classifying leukocytes, and reagent for classifying leukocytes

The sample preparation apparatus includes a measurement unit configured to measure a sample containing particles acquired from a sample container and detect measurement target particles in the sample, a sample preparation unit capable of adjusting the concentration of measurement target particles in a sample acquired from a sample container and configured to prepare a measurement sample by mixing a sample and any of a plurality of types of particle detection reagents including a particle labeling substance, and a control unit for controlling the sample preparation unit so as to generate concentration information of the measurement target particles in the sample in the sample container based on the measurement data of the measurement unit and adjust the concentration of the measurement target particles in the sample acquired from the sample container according to the generated concentration information and the type of particle detection reagent used for preparing the measurement sample.

Sample preparation apparatus, sample preparation system, sample preparation method, and particle analyzer

The sample preparation apparatus includes a measurement unit configured to measure a sample containing particles and detect measurement target particles in the sample, a sample preparation unit capable of adjusting the concentration of measurement target particles in a sample and configured to prepare a measurement sample by mixing a sample and a plurality of types of particle detection reagents, and a control unit configured to generate concentration information of the measurement target particles in the sample based on the measurement data of the measurement unit and adjust the concentration of the measurement target particles in the sample according to the generated concentration information and the type of particle detection reagent.

A blood analyzer comprising: a specimen preparation section for preparing a measurement specimen that is used to measure a white blood cell count among CBC measurement items; a measurement section for obtaining optical information from blood cells contained in the measurement specimen; and a controller carrying out operations comprising: classifying blood cells contained in the measurement specimen into at least white blood cells and blood cells suspected to be abnormal blood cells based on the optical information; obtaining distribution data of the white blood cells and distribution data of the blood cells suspected to be abnormal blood cells; counting the white blood cells based on the distribution data of the white blood cells; and determining presence or absence of the abnormal blood cells in the blood sample, based on the distribution data of the blood cells suspected to be abnormal blood cells. A blood analyzing method and a computer program product are also disclosed.

Blood analyzer, blood analyzing method, and computer program product

The present invention provides a reagent for analysis of immature leukocytes comprising: 

Reagent and reagent kit for analysis of immature leukocyte

The present invention provides a reagent kit for analyzing a sample comprising a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid and a second reagent containing a fluorescent dye capable of staining nucleic acid, and a method for analyzing a sample using the kit.

Reagent kit for sample analysis and sample analysis method

The present invention provides a reagent for analysis of immature leukocytes comprising:

An apparatus for measuring immature platelets is described that includes (a) a sample preparation unit for preparing an assay sample by adding a reagent to a blood specimen; (b) a detection unit having a semiconductor laser light source for irradiating the assay sample with laser light, and a detector for detecting optical information emitted from particles within the assay sample irradiated by laser light; and (c) a controller for differentiating and counting immature platelets based on the detected optical information. A method for measuring immature platelets is also described.

Apparatus and method for measuring immature platelets

A measuring method and a measuring apparatus which can classify and count myeloblast more precisely without influence of other component in a sample including platelet aggregation in measurement of a blood sample with a flowcytometry, wherein damage is given to a cell membrane of erythrocyte and mature leukocyte contained in a hematological sample, a hemocyte in which a cell membrane is damaged is constricted, and this is dyeing-treated with a fluorescent dye which can stain a nucleic acid to obtain a sample, the sample is measured with a flowcytometer, and a cell contained in a first cell group containing myeloblast, which is specified based on forward scattered light information and side scattered light information, and contained in a second cell group containing myeloblast, which is specified based on forward scattered light information and fluorescent information, is counted as myeloblast.

Method and apparatus for measuring hematological sample

A sample analyzer including a detection unit for irradiating a biological sample with light and obtaining optical information; a cell classification processor for classifying cells contained in the biological sample into cell groups based on the optical information; a scattered light information obtaining processor for obtaining scattered light information relating to a cell included in a predetermined cell group; a calculation processor for calculating a component value corresponding to an amount of component contained in cell included in the predetermined cell group based on the scattered light information; and an output device for outputting the component value calculated by the calculation means is disclosed. A computer program product is also disclosed.

Sample analyzer and computer program product

A reagent for analyzing immature leukocyte capable of analyzing accurately immature leukocyte such as myeloblast and immature granulocyte, and mature leukocyte, which reagent has broader allowable range of sample processing conditions than conventional ones, and a reagent kit are provided. The present invention relates to a reagent for analyzing immature leukocyte containing a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte in the sample, a solubilizing agent for causing contraction to damaged blood cell, sugar, a dye for staining nucleic acid.

Reagent for immature leukocyte analysis and reagent kit

A cell analysis apparatus includes a test sample preparation part for preparing a test sample by treating an analyte with a dye for staining an intracellular substance of cells in the analyte, a detector for detecting scattered light and fluorescence from each cell in the test sample, and a controller for identifying living mononuclear cells based on the scattered light and the fluorescence detected by the detector. A cell analysis method includes preparing a test sample by treating the analyte with a dye for staining an intracellular substance of cells in the analyte; detecting scattered light and fluorescence from each cell in the test sample; and identifying living mononuclear cells based on the scattered light and the fluorescence detected.

Growing community of inventors

Kobe, Japan

Shinichiro Oguni