Shimadzu Corporation

Shinichiro Kobayashi

Masamitsu Shikata

Nobuhiro Hanafusa

Naoko Takaoka

Kenji Ninomiya

A dispensing tip holder is used when a dispensing tip is attached to a tip of a pipette, and includes a peripheral wall having a shape surrounding an outer peripheral surface of the dispensing tip, and an insertion portion having an insertion opening into which the dispensing tip is to be inserted. The peripheral wall has a first end into which the dispensing tip is to be inserted, and has a second end provided at a position opposite to the first end. The insertion portion is formed in the peripheral wall such that an outer peripheral surface of the dispensing tip abuts against an edge of the insertion opening, and is formed in the peripheral wall such that a tip of the dispensing tip does not project from the second end of the peripheral wall, when the dispensing tip is inserted into the insertion opening from the first end.

Dispensing tip holder

The present invention provides a simple cell-free protein synthesis method capable of affording synthesis of a protein in a high amount in a short time at a low cost. A method for cell-free protein synthesis using an extract derived from an insect cell, the method comprising removing a component which can pass through a semipermeable membrane through the semipermeable membrane while maintaining synthesis reaction, thereby to continuously synthesize a protein. Preferably, an mRNA is additionally supplied while said synthesis reaction is maintained. Further, said insect cell is preferably an established culture cell derived fromovum cell.

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Kyoto, Japan

Shinichiro Kobayashi