Fuji Yakuhin Kogyo Kabushiki Kaisha

Shinichi Yoshida

Kazushi Iwata

Akira Oshima

Yasuo Bai

Yasuteru Muragaki

Eiji Ishikawa

Kazuyoshi Inada

Yasuo Nagai

Kenichi Obata

The present invention is directed to a method for detecting hepatic diseases which are associated with fibrosis by determining the level of human prolyl hydroxylase in a serum sample which comprises:

Method for determining beta-subunit of human prolyl 4-hydroxylase by

A method for determining human prolyl hydroxylase by radioimmunoassay according to the sandwich method wherein a monoclonal antibody to human prolyl hydroxylase and polyclonal antibody to human prolyl hydroxylase are used, characterized in that a monoclonal antibody to human prolyl hydroxylase is used as at least one of the antibodies which are to be coated on a solid support and to be labeled with a radioactive element. This method is simple and operable with small amounts of samples and gives exact results. Thus, this method is useful for the diagnosis of hepatic diseases. A monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase is used to test for the human prolyl hydroxylase.

Method for determining the .beta.-subunit of human prolyl hydroxylase by

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Toyama, Japan

Shinichi Yoshida