Ajinomoto Co., Ltd.

Shinichi Sugimoto

Hisashi Yasueda

Yoshihiro Usuda

Yoshio Kawahara

Yousuke Nishio

Ryo Takeshita

Kazuhiko Matsui

Osamu Kurahashi

Tsuyoshi Nakamatsu

Eiichiro Kimura

Yumi Matsuzaki

Nobuharu Tsujimoto

Seiko Hirano

Gen Nonaka

Kazue Kawamura

Tsuyoshi Osumi

Chie Koseki

Naoki Akiyoshi

Kanae Nakamura

Isamu Shiio

Yoshiya Gunji

Mayu Iyo

Megumi Shimaoka

Masahiro Kakehi

Atsushi Yokota

Yukiko Ono

Yukiko Tabira

Yuri Miyata

Manami Oba

Yasuhiko Toride

A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.

Method for producing basic substance

An L-amino acid is produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the activity of an iron transporter is increased by enhancing expression of one or more genes of the following genes: tonB gene, fepA gene, and fecA.

L-amino acid producing bacterium and method of producing L-amino acid

L-amino acid is produced by culturing a bacterium belonging to the Enterobacteriaceae family which has L-amino acid-producing ability and is modified so that expression of the nhaA gene, nhaB gene, nhaR gene, chaA gene, mdfA gene, or combinations thereof is enhanced.

L-amino acid producing bacterium which has enhanced expression of at least one of the nhaA gene, the nhaB gene, the nhaR gene, the chaA gene, the mdfA gene and a method of producing L-amino acid

The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacteriumssp.and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

[object Object]

Nucleoside 5'-phosphate ester is produced by culturing a bacterium belonging to the genushaving an ability to produce nucleoside 5&#x2032;-phosphate ester, in which ushA gene and aphA gene do not function normally, in a medium to produce and accumulate nucleoside 5&#x2032;-phosphate ester in the medium, and collecting the nucleoside 5&#x2032;-phosphate ester from the medium.

Method for producing nucleotide by fermentation

A plasmid isolatable from, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

Plasmid autonomously replicable in coryneform bacteria

An L-amino acid is produced by culturing abacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, abacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or abacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.

The present invention provides polypeptides and polynucleotides involved in amino acid biosynthesis inand methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Growing community of inventors

Kawasaki, Japan

Shinichi Sugimoto