Toyota Jidosha Kabushiki Kaisha

Shinichi Ohnuma

Tokuzo Nishino

Chikara Ohto

Kazutake Hirooka

Chika Asada

Yoshie Takeuchi

Hisashi Hemmi

Hiroyuki Nakane

Chika Ishida

Keishi Narita

Shusei Obata

Tanetoshi Koyama

Kyozo Ogura

Ayumi Koike

Takeshi Nakazawa

Yuka Higuchi

Manabu Suzuki

A mutant prenyl diphosphate synthase capable of synthesizing prenyl diphosphates, shorter than those synthesized by the original enzyme, by modifying the amino acid sequence in and upstream of the aspartic acid-rich domain DDXX (XX)D (X denotes any amino acid, and XX in the parentheses may not be present) present in region II of the prenyl diphosphate synthase.

Mutant prenyl diphosphate synthase

A mutant prenyl diphosphate synthase having a modified amino acid sequence of prenyl diphosphate synthase, wherein the amino acid residue located at the eighth position in the N-terminal direction from D of the N-terminal of the Asp-rich domain DDXX(XX)D (in the sequence X denotes any amino acid and the two X's in the parentheses may not be present) present in the region II has been substituted by another amino acid, or both of the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal and the amino acid residue located at the eighth position in the N-terminal direction from D of the N-terminal have been substituted by other amino acids.

Mutated prenyl diphosphate synthases

Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.

Mutant prenyl diphosphate synthase, DNA encoding mutant prenyl diphosphate synthase and process for producing mutant prenyl phosphate synthase

A mutant prenyl diphosphate synthase capable of synthesizing prenyl diphosphates, shorter than those synthesized by the original enzyme, by modifying the amino acid sequence in and upstream of the aspartic acid-rich domain DDXX(XX)D (X denotes any amino acid, and XX in the parentheses may not be present) present in region II of the prenyl diphosphate synthase.

Farnesyl diphosphate synthase

The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.

Process for the production of prenyl diphosphate using mutants of

The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein, at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.

Nucleic acid encoding mutant geranylgeranyl diphosphate synthase

Long-chain prenyl diphosphate synthase

DNA coding for thermostable geranylgeranyl diphosphate (GGDP) synthase derived from Sulfolobus acidocaldarius is provided. The DNA is useful for production of GGDP synthase, which is, in turn, useful for production of GGDP.

Geranylgeranyl diphosphate synthase and DNA coding therefor

A mutated farnesyldiphosphate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding for said mutated enzyme, wherein the mutated enzyme is modified from a native farnesyldiphosphate synthase by mutation of a gene coding for a native farnesyldiphosphate synthase.

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Sendai, Japan

Shinichi Ohnuma