Tokyo Electron Limited

Sinfonia Technology Co., Ltd.

Tocalo Co., Ltd.

Shinichi Gomi

Kenichi Kagawa

Shigenori Ozaki

Yasuhiro Oshima

Tomoaki Kurakazu

Yoshio Kimura

Yusuke Yoda

Tatsuya Hamaguchi

Shinya Miki

Kunitada Hatabayashi

Kentaro Tomita

The purpose of the present invention is to provide a culture container capable of preventing a concave meniscus without using a jig. In order to achieve this purpose, the present invention provides a culture container (A) provided with: a base () having a concave portion (); and a water-repellent layer () formed on an outer edge region () of the bottom () of the concave portion () and the inner circumferential surface () of the concave portion (). One of the surfaces of the water-repellent layer () is exposed to the space in the concave portion ().

Culture container and cell culturing method and cell observation method using culture container

There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

Method for determining undifferentiated state of pluripotent stem cells by culture medium analysis

A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

Cell culture container

Growing community of inventors

Tokyo, Japan

Shinichi Gomi