Toray Industries, Inc.

Riken Corporation

Shingo Hiramatsu

Katsushige Yamada

Yusuke Kagawa

Koji Kobayashi

Haruka Saito

Kentaro Ishii

Masashi Higasa

Kenji Sawai

Kyohei Isobe

Chiaki Yamada

Takashi Tanaka

Moriya Ohkuma

Shigeharu Moriya

Masahiro Yuki

Shiomi Watanabe

Kyungsu Na

Masato Otagiri

Toshiki Tamura

A mutant strain of, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6. A method produces a cellulast, the method including a step of cultivating the mutant strain of, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6.

Mutant strain ofand method of producing protein using same

A mutant of afilamentous fungus has a mutation that results in lost or lowered function of a beta-adaptin large subunit, or a mutation in an amino acid sequence that forms the beta-adaptin large subunit. A method produces a protein while maintaining a low viscosity in a culture solution during culturing by using the mutant.

Mutant offilamentous fungus and method of producing protein

A mutant strain ofhas a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2. A method produces a protein, the method including a step of cultivating the mutant strain.

mutant strain, and method of producing protein

A mutant strain of a filamentous fungus of the genushaving a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2; and a method of producing a sugar from a cellulose-containing biomass, the method including: step a of producing a cellulase by cultivating amutant strain having a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2, and step b of saccharifying the biomass by using the cellulase obtained in the step a.

Trichoderma filamentous fungus mutant strain and method of producing protein

Amutant strain has a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 is reduced. A method of producing a protein includes a step of cultivating the-mutant strain, and a method of producing a cellulase includes a step of cultivating themutant strain.

Trichoderma reesei mutant and protein production method

A method produces a protein using a filamentous fungus, in which decrease in dissolved oxygen saturation during culture of the filamentous fungus can be suppressed even when the culture is scaled up. The method of producing a protein includes culturing a fungus belonging to the genuswhose BXL1 gene was disrupted, using a biomass containing cellulose and xylan as an inducer. The use of the BXL1 gene-disrupted fungus belonging to the genusenables suppression of the decrease in dissolved oxygen saturation even when xylose and cellulose are used as inducers.

Method of producing protein

The object of the present invention is to separate and provide a &#x3b2;-glucosidase gene having the effect of efficiently promoting saccharification in hydrolysis of cellulose-containing biomass from a hardly culturable symbiotic protist community of, and the present invention specifically relates to &#x3b2;-glucosidase derived from a protist of the genusconsisting of the amino acid sequence represented by SEQ ID NO: 1.

Beta-glucosidase, enzyme composition including same, and method for manufacturing sugar solution using same

A novel fungus belongs to the genuswhose cellulase can be used to hydrolyze cellulose-based biomass without degradation of xylan contained in the biomass into xylose, and a method produces glucose and xylo-oligosaccharides from a cellulose containing biomass using it. The fungus belonging to the genusincludes N- and C-terminal domains of the &#x3b2;-xylosidase 1 (BXL1) gene, but lacks the Fn3-like domain of the gene due to its disruption. The use of this fungus belonging to the genusresults in deletion of &#x3b2;-xylosidase activity and increase in &#x3b2;-glucosidase activity. Thus, in hydrolysis of cellulose contained in biomass, xylan contained in the biomass is not degraded into xylose, which enables efficient production of glucose and xylo-oligosaccharides.

fungus having mutant-type BXL1 gene and method of producing xylooligosaccharide and glucose by using same

A method produces a chemical product by continuous fermentation including filtering a culture liquid of a microorganism(s) through a separation membrane, retaining unfiltered liquid in, or refluxing unfiltered liquid to, the culture liquid, adding a fermentation feedstock to the culture liquid, and recovering a product in the filtrate, wherein the microorganism(s) is/are a microorganism(s) that undergo(es) catabolite repression, and the fermentation feedstock comprises hexose and pentose.

Method of producing chemical substance

A liquid-feeding chip for feeding a liquid utilizing the action of centrifugal force and gravity by rotating the chip around an axis of rotation, includes a first storage tank (-) into which the liquid can be introduced when rotation of the chip is stopped, and two or more liquid-feeding units arranged in a plurality of levels adjacent to each other, each liquid-feeding unit (U-U-U-) being composed of a first holding tank (---), a second holding tank (---) positioned in the direction of gravity with respect to the first holding tank, and a channel B (B-B-B-) which extends from the first holding tank in the direction of gravity and which connects the first holding tank and the second holding tank, the first holding tank at a first level being connected with a channel A (A-) which extends from the first storage tank toward an outer circumferential side. In the liquid-feeding chip, the adjacent liquid-feeding units are connected by a channel C (C-C-) which extends from the second holding tank of the liquid-feeding unit at an upper level to an outer circumferential side during rotation, and which is in communication with the first holding tank of the liquid-feeding unit at a lower level.

Liquid-feeding chip and analysis method

A separation chip for separating an insoluble component from a suspension using centrifugal force by rotation, includes a suspension holding tank, a separation liquid holding tank, and an insoluble component holding tank arranged in order from an inner circumferential side during rotation, wherein the suspension holding tank and the insoluble component holding tank are connected to each other, the insoluble component holding tank and the separation liquid holding tank are connected to each other by a narrow portion, and in the insoluble component holding tank, a connecting portion with the suspension holding tank is positioned further toward an outer circumferential side than the narrow portion.

Separation chip and separation method

The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3' terminal portion and the DNA sequence of the 5&#x2032; terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.

Growing community of inventors

Kamakura, Japan

Shingo Hiramatsu