Centre National De La Recherche Scientifique

Cnrs

Shingo Fujii

Robert Fuchs

Philippe Dumas

Vincent Olieric

Dominique Yves Joel Burnouf

Jerome Edouard Wagner

Robert Pierre Paul Fuchs

The invention is to supply a novel way for the isolation and identification of proteins bound to any kind of interesting nucleic acid sequence (Sequence-of-Interest: SoI), advantageously to any kind of interesting DNA sequence, particularly in the context of chromosomal DNA or RNA or episomal DNA in living cells or in test tubes. In the context of the present invention, living cells include any organism that contains nucleic acid material as for example viruses, bacteria, cells, the bound protein of which have to be analyzed. The invention is based upon the use of a specific nucleic acid sequence tag, advantageously a specific double-stranded DNA able to form triplex helix, referred to as the Triplex-Forming Tag sequence, (TFT sequence), that will be located nearby the SoI.

Method for the isolation of proteins binding to any kind nucleic acid sequence of interest

A protein crystal having the processivity clamp factor of DNA polymerase that is the &#x3b2; subunit of DNA polymerase III ofand a peptide of about 3 to about 30 amino acids, in particular of about 16 amino acids. The peptide includes all or part of the processivity clamp factor binding sequence of a processivity clamp factor interacting protein, such as prokaryotic Pol I, Pol II, Pol III, Pol IV, Pol V, MutS, ligase I, &#x3b1; subunit of DNA polymerase, UmuD or UmuD', or eukaryotic pol &#x3b5;, pol &#x3b4;, pol &#x3b7;, pol &#x3b9;, pol &#x3ba;.

Protein crystal comprising the processivity clamp factor of DNA polymerase and a ligand, and its uses

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Marseilles, France

Shingo Fujii