The University of Tokyo

Kisco

Daisan Kasei Co., Ltd

Shin Ohya

Teruo Fujii

Yasuyuki Sakai

Takashi Inoue

Takatoki Yamamoto

Masaki Nishikawa

Hitomi Sakai

Hirosuke Naruto

The invention is directed to methods for the propagation or cultivation of cells including preparing a cell culture substrate, wherein the cell culture substrate includes a substrate and a layer formed by surface modification. The layer includes a polymer containing an amino group. The polymer is produced by reacting a polymer represented by formula (II): with a polymer having at least one amino group, &#x2014;NH, capable of forming a Schiff base in a monomer of formula (II), thereby forming a polymer layer constituting the layer formed by surface modification. 'n' in Formula (II) is 0 or a positive integer, and m is a positive integer. n and m represent the degree of polymerization. Formula (II) is formed by chemical vapor deposition of formyl[2.2]paracyclophane. The methods further include providing cells in a medium; inoculating the cells onto the cell culture substrate; and culturing the cells, wherein the cells adhere to the cell culture substrate.

Cell culture substrate and its production method

Growing community of inventors

Chuo-ku, Japan

Shin Ohya