Kyoto University

Takeda Pharmaceutical Company Limited

Kirin Holdings Kabushiki Kaisha

National Cancer Center Japan

Thyas Co. Ltd.

Shin Kaneko

Atsutaka Minagawa

Yasushi Uemura

Yohei Kawai

Yoshiaki Kassai

Tatsuki Ueda

Shoichi Iriguchi

Kyoko Fukuda

Norihiro Ueda

Yutaka Yasui

Tetsuya Nakatsura

Shinichiro Takayanagi

Kyoko Masuda

Akitsu Hotta

Hiroshi Kawamoto

Akira Hayashi

Ken Fukumoto

Hiroshi Ichise

Yutaka Shimazu

Kazuhide Nakayama

Atsushi Matsuda

Kazuki Nakazono

Sayaka Chuganji

Atsushi Kunisato

Toshiaki Yoshikawa

Suguru Arima

Maiko Takiguchi

The present invention provides a method for producing a γδT cell from an induced pluripotent stem cell, wherein the induced pluripotent stem cell is derived from a cell other than an αβT cell.

Method for producing gamma delta T cells

Provided is a method for inducing T cells for a cell-based immunotherapy, comprising the steps of:

Method for inducing t cells for cell-based immunotherapy from pluripotent stem cells

The present invention relates to a method for producing T cells or NK cells including culturing T cells or NK cells in a culture medium containing a CaMKII inhibitor such as a bisbenzylisoquinoline alkaloid compound. The disclosed compounds and methods can be used for maintaining the undifferentiated state of undifferentiated T cells, or for efficiently proliferating T cells or NK cells.

Production method for T cells or NK cells, medium for culturing T cells or NK cells, method for culturing T cells or NK cells, method for maintaining undifferentiated state of undifferentiated T cells, and growth-accelerating agent for T cells or NK cells

A method efficiently produces cytotoxic T lymphocytes having intrinsic properties of lymphocytes of the acquired immune system suitable for cellular immunotherapy. The method includes culturing CD4/CD8 double-positive T cells in a medium containing IL-7 and a T-cell receptor activator, to induce CD8αβcytotoxic T lymphocytes.

Method for producing CD8alpha+beta+ cytotoxic t cells

An object of the present invention is induction of CD4-positive T cells from pluripotent stem cells. This object is achieved by production of CD4-positive T cells by introducing a CD4 gene or a gene product thereof into T cells induced from pluripotent stem cells.

Method for producing CD4-positive T cells from pluripotent stem cells

Provided is a T cell receptor capable of binding to a peptide having the amino acid sequence shown in SEQ ID NO: 27 or a complex of the peptide and HLA-A24. A T cell receptor capable of binding to a peptide having the amino acid sequence shown in SEQ ID NO: 28 or a complex of the peptide and HLA-A02. Disclosed T cell receptors are useful in treating or avoiding cancers which are associated with expression of glypican-3.

T-cell receptor

A method for producing CD4/CD8 double-positive T cells, comprising the steps of: (1) culturing pluripotent stem cells in a medium to induce hematopoietic progenitor cells; and (2) culturing the hematopoietic progenitor cells obtained in the step (1) in a medium containing a p38 inhibitor and/or SDF-1 to induce CD4/CD8 double-positive T cells.

Method for producing CD4/CD8 double-positive T cells

A method of producing helper T cells, comprising:

Method for producing helper T cells from pluripotent stem cells

The present invention provides a method for efficiently inducing CD8-positive T cells by adding vitamin C to the medium in the steps of induction of the CD8-positive T cells from pluripotent stem cells. The present invention also provides a method for efficiently inducing CD8-positive T cells by performing culture in a medium supplemented with an adrenocortical hormone agent in the step of induction of the CD8-positive T cells from CD4/CD8 double-positive T cells.

Method for induction of T cells from pluripotent stem cells

Growing community of inventors

Kyoto, Japan

Shin Kaneko