Kyowa Hakko Bio Co., Ltd.

Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Kogyo

Shin-ichi Hashimoto

Yoshiyuki Yonetani

Hajime Ikeda

Kazuhiko Tabata

Akio Ozaki

Makoto Yagasaki

Hiroshi Mizoguchi

Hirofumi Endo

Ryoichi Katsumata

Kenji Soda

Hideo Kawabe

Keiko Ochiai

Koji Morishita

Yasushi Sakai

Toshikazu Kamiya

Koji Harada

Masaki Maeda

Mikiro Hayashi

Haruhiko Yokoi

Miho Komatsu

Koichiro Miyake

Masao Kimura

Shun Kayahashi

Akihiro Senoo

Seiko Ando

Tetsuo Nishimura

Yugo Adachi

Aya Kubota

Nozomu Kamada

Tetsuya Nishitani

Aya Hadakubota

Shizuo Tsuchiya

A muscle fatigue remedy that for persons with the symptoms of muscle pain attributed to strenuous exercise, lassitude in the arms and legs attributed to acute exercise, lower back pain and stiffness in the shoulder attributed to taking of a certain posture over a prolonged period of time, etc., relieves the symptoms and allows them to have a fulfilling life. There is provided a muscle fatigue remedy containing alanylglutamine or its salt as an active ingredient.

Muscle fatigue remedy

The invention provides a microorganism belonging to Enterobacteriaceae in which a function of CsrC RNA has been decreased or lost, and which has the ability to produce and accumulate an amino acid, and a process in which the microorganism is cultured in a medium to produce and accumulate the amino acid in the culture, and the amino acid is recovered from the culture.

Method for production of amino acid

The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5'-monophosphate, adenosine-5&#x2032;-diphosphate and adenosine-5&#x2032;-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase activity, or a culture of cells having the ability to produce the protein or a treated matter of the culture, and a protein having the activity to ATP-dependently form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture.

Process for producing dipeptides or dipeptide derivatives

The present invention provides: (1) crystals of a dipeptide which do not substantially comprise a dipeptide comprising D-amino acid as a constituent or a polypeptide consisting of three or more amino acids; and (2) crystals of a dipeptide which do not substantially comprise a dipeptide comprising D-amino acid as a constituent, a polypeptide consisting of three or more amino acids, or an amino acid amide; and a process for producing the dipeptide crystals.

L-alanyl-L-glutamine crystal

The present invention provides a microorganism belonging to the genusin which the activities of glutamine-synthetase adenylyltransferase (GlnE protein) and PII regulatory protein for glutamine synthetase are reduced or lost and which has the ability to form and accumulate L-glutamine or a substance biosynthesized utilizing nitrogen supplied by L-glutamine, and a process for producing L-glutamine or the substance biosynthesized utilizing nitrogen supplied by L-glutamine using the microorganism.

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According to the present invention, it is possible to provide a microorganism belonging to the genus, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.

Method for production of L-glutamine

The present invention provides a protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for proteins of the amino acid sequences as shown in SEQ ID NOS: 4 to 6), and a process for industrially advantageously producing a compound that inhibits HMG-CoA reductase and has an action to decrease serum cholesterol, using DNA encoding the protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for DNA encoding the protein comprising the amino acid sequence as shown in SEQ ID NO: 4).

Protein and DNA encoding the protein

The present invention provides a protein which catalyzes the synthesis of a dipeptide different from L-Ala-L-Ala, a process for producing the protein which catalyzes the synthesis of a dipeptide, a process for producing a dipeptide using the protein which catalyzes the synthesis of a dipeptide, and a process for producing the dipeptide using a culture of a microorganism producing the protein which catalyzes the synthesis of a dipeptide or the like as an enzyme source.

Process for producing dipeptides

The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least one of said one or more kinds of amino acids, allowing the dipeptide to form and accumulate in the medium, and recovering the dipeptide from the medium.

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,

Process for producing HMG-CoA reductase inhibitor

A composition for oral administration is provided, which includes at least one dipeptide represented by the formula: (wherein X represents alanyl, and Y represents valine, leucine or isoleucine), with an object of providing a composition for oral administration which is excellent in nutrition, pharmacological effect and gustation, or providing a composition for oral administration which is excellent in processing characteristics such as solubility and tableting property.

Dipeptide-comprising composition for oral administration

The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.

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Hofu, Japan

Shin-ichi Hashimoto