The United States of America As Represented by the Secretary of

Toyotama Perfumery Co., Ltd.

Shin Hasegawa

Yasushi Ifuku

Hisao Maeda

Luke K Lam

Kazuyuki Maruyama

Nobuya Inaba

Masaki Miyake

Shigeru Ayano

Yoshihiko Ozaki

A method for manufacturing limonoid glucosides that is superior in terms of industrial productivity is described. The method includes a step in which a raw material composed of citrus juice and/or molasses is passed through a flow passage equipped with an adsorbent having limonoid glucoside adsorptivity, after which the limonoid glucosides that have been adsorbed by the adsorbent in the flow passage are eluted into a solvent using a solvent capable of dissolving the limonoid glucosides, which yields a liquid containing limonoid glucosides.

Method for manufacturing limonoid glucosides

A specified citrus limonoid such as limonin and nomilin is isolated from the citrus and is found to be useful to treat a tumor. It is useful as an additive to food.

Antitumor agent

Bitterness in limonoid-containing citrus juice is reduced by treatment with a novel strain of Corynebacterium fascians having the capability of producing enzymes for metabolizing limonoids without the presence of a limonoid inducer in the growth medium.

Strain of Corynebacterium Fascians and use thereof to reduce limonoid

Growing community of inventors

Pasadena, CA, United States of America

Shin Hasegawa