Kyoto University

Shimpei Gotoh

Michiaki Mishima

Yuki Yamamoto

Satoshi Konishi

Kenji Osafune

Isao Ito

This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3&#x3b2; inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF&#x3b2; inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3&#x3b2; inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3&#x3b2; inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.

Method for inducing differentiation of alveolar epithelial cells

This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3&#x3b2; inhibitor, (2) a medium containing a BMP inhibitor and a TGF&#x3b2; inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3&#x3b2; inhibitor.

Method for inducing alveolar epithelial progenitor cells

This invention provides a method for stably producing airway epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing airway epithelial cells from pluripotent stem cells comprising steps: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3&#x3b2; inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF&#x3b2; inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3&#x3b2; inhibitor; (5) subjecting the cells obtained after Step (3) to three-dimensional culture in a medium containing a GSK3&#x3b2; inhibitor, FGF10, and a ROCK inhibitor; and (6) subjecting the proximal airway epithelial progenitor cells obtained in Step (5) to three-dimensional culture in a medium containing a ROCK inhibitor.

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Kyoto, Japan

Shimpei Gotoh