Hubei University

Shihui Yang

Qiaoning He

Yu Wang

Yi Wang

Lixin Ma

Wei Shen

Runxia Li

Yunhao Chen

Yongfu Yang

Ju Huang

Yueyue Chen

Mian Li

Li Yi

Xia Wang

Xiongying Yan

Mimi Hu

Genetically modifiedto utilize inorganic N source, methods and uses thereof are provided. The genetically modifiedhas a genome with the knockout of a locus ZMO1107 fromZM4 could ferment inorganic N source.

Genetically modifiedto utilize inorganic n source, methods and uses thereof

Recombinantfor producing ethylene glycol, method and uses thereof are provided. The recombinantcarries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

Recombinantfor producing ethylene glycol, method and uses thereof

A construction method of a regulation system for gene expression inand applications are provided. The T7 expression system inhas been constructed. The T7 expression system is used to construct a regulation circuit, help analyze the function of toxic genes and realize the regulation of metabolic pathways in. At the same time, shuttle plasmids that can be used inand, also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.

Construction method of a tight regulation system for gene expression inand applications

A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of, so as to be applied in metabolic engineering of different demands.

Method for characterizing biological part based on dual-fluorescent reporter gene system and biological part library constructed thereon

Growing community of inventors

Hubei, China

Shihui Yang