Monsanto Technology LLC

University of Missouri

Shihshieh Huang

Larry A Gilbertson

Thomas M Malvar

Edwards M Allen

Michael W Petersen

Xudong Ye

Elysia K Krieger

Michael H Luethy

James Kevin Roberts

Sara Elizabeth Heisel

David A Walters

Paul S Chomet

Susan Jane Johnson

Nancy M Houmard

Sergey Ivashuta

Brian J Martinell

Qi Wang

Kenneth A Barton

Yong Huang

Kenneth John Piller

Toni Armstrong

Laura J Crow

Alessandra Frizzi

Yuanji Zhang

Liang Guo

Stanislaw Flasinski

David K Kovalic

Barry S Goldman

Lyle Dean Crossland

Nordine Cheikh

Brad Kane Gabor

Roy O Morris

Alberto Iandolino

John Kao

Gregory John Peel

Charles Hagen

Raquel Salati

David N Walters

Linda L Lutfiyya

Alan L Kriz

Philip W Miller

Michael J Bennett

Janette V Oakes

Steven H Schwartz

Wayne Brown

Sharon E Radke

David K Kovalik

Mike Kerns

Heather A Lindfors

Susan Johnson

Alberto B Iandolino

Sergey I Ivashuta

Yuanji I Zhang

Sara E Heisel

David E Kovalic

Yaunji Zhang

Michael H Leuthy

The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

DNA constructs for obtaining marker-free transgenic plants

Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.

Recombinant DNA constructs employing site-specific recombination

The present disclosure provides compositions and methods for delivering a polynucleotide from the exterior surface of a plant or plant part into the interior of a plant cell. More specifically, the present disclosure relates to compositions comprising at least one polynucleotide and at least one agent that is able to disrupt at least one barrier of the plant or plant part.

Compositions and methods for delivery of a polynucleotide into a plant

This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

Methods for producing hybrid seed

The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Recombinant DNA constructs and methods for controlling gene expression

Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5' to 3&#x2032; order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.

Enhanced zein reduction in transgenic corn seed

The present disclosure provides compositions and methods for introducing nucleic acids into plants. More specifically, disclosed herein are methods and compositions for introducing a nucleic acid, such as a double-stranded RNA, for silencing a target gene in a plant. Also disclosed herein are plants exhibiting modification of a target gene, obtained through the use of such methods and compositions.

Methods and compositions for introducing nucleic acids into plants

This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

Plant microRNAs and methods of use thereof

Methods and compositions for obtaining marker-free transgenic plants

Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5' to 3&#x2032; order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.

Recombinant DNA for gene suppression

The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.

Multiple virus resistance in plants

Growing community of inventors

Woodland, CA, United States of America

Shihshieh Huang