Arizona State University

Shih-Hui (Joseph) Chao

Deirdre Meldrum

Laimonas Kelbauskas

Jeff Houkal

Roger H Johnson

Hong Wang

Andrew Hatch

Rishabh Shetty

Jakrey Myers

Weimin Gao

Iniyan Soundappa Elango

David Wayne Richardson

Dean Smith

Brian Ashcroft

Thai Tran

Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.

Live-cell computed tomography

Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.

System and method for laser lysis

Growing community of inventors

Phoenix, AZ, United States of America

Shih-Hui (Joseph) Chao