National Health Research Institutes

Industrial Technology Research Institute

Shin Kong Wu Ho Su Memorial Hospital

Shih-Feng Tsai

Wei-Ming Chen

Ping-Fu Cheng

Tzu-Ling Tseng

Yu-Fen Liu

Horng-Yunn Dou

Chien-Hsing Lin

Pei-Hsiu Liao

Hou-Chang Chiu

The present application provides a method for genotyping, comprising obtaining amplifying and obtaining a first DNA fragment from a DNA sample by using one or more primer sets selected from the group consisting of primer sets 1 to 25 (SEQ ID Nos. 1 to 50); amplifying and obtaining a second DNA fragment from the obtained first DNA fragment by using one or more extension primers selected from the group consisting of SEQ ID Nos. 51 to 75; and detecting the second DNA fragment by using mass spectrometry, particularly by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

Method for genotyping

The present invention relates to methods of diagnosing myasthenia gravis in a subject, by determining an amount of at least one autoantibody that specifically binds one or more autoantigens selected from heat-shock protein 60 (hsp60), heat-shock protein 90, alpha isoform (hsp90&#x3b1;), and heat-shock protein 90, beta isoform (hsp90&#x3b2;). The invention also provides diagnostic kits for identifying a subject having myasthenia gravis.

Method of diagnosing myasthenia gravis and kits therefor

Methods, compounds, and kits for the diagnosis or screening of osteonecrosis are described, and the development of animal models for COL2A1 function in osteonecrosis is put forth. Novel mutations in the COL2A1 gene are identified that are associated with avascular necrosis of the femoral head. Methods of treatment of osteonecrosis and avascular necrosis of the femoral head, including gene therapy approaches comprising introduction of COL2A1 nucleic acid are contemplated.

Methods and compositions relating to COL2A1 gene mutations and osteonecrosis

Growing community of inventors

Miaoli County, Taiwan

Shih-Feng Tsai