Ajinomoto Co., Ltd.

Shigezo Udaka

Other

Takeda Chemical Industries, Inc.

Nagoya University

Sumitomo Pharmaceuticals Company Limited

Hoechst Japan Limited

Nihon Shokuhin Kako Co., Ltd.

Hideo Yamagata

Shigeyoshi Miyashiro

Tsuyoshi Shiio

Norihiro Tsukagoshi

Susumu Iwasa

Toshihiko Ando

Takao Kida

Hiroshiro Shibai

Kazuo Hirayama

Takeo Kato

Kenji Sakaguchi

Hiroko Tada

Asao Murai

Atsushi Tsujimura

Yasushi Inoue

Tsutomu Kajino

Yukio Yamada

Masana Hirai

Yoko Saito

Noriyuki Kitamoto

Fumihiko Hoshino

Makoto Shoda

Eikou Shimizu

Tamotsu Hashimoto

Koen Dekker

Process for production of human growth hormone using Bacillus Brevis

A bacterium of the genus Bacillus is transformed by introducing a vector incorporating DNA encoding an antibody; the resulting transformant is cultivated in a medium to produce and accumulate the antibody in an active form in the culture liquid. According to this method, a recombinant antibody can be easily produced in an active form in large amounts, owing to success in secretory expression using a bacterium of the genus Bacillus.

Antibody-encoding recombinant DNA and its use

A xylose isomerase gene from Thermus bacteria, such as Thermos aquaticus (ATCC 27634) and a gene having 60% or more of homology to the nucleotide sequence of Thermus aquaticus xylose isomerase gene of FIG. 1-3. A xylose isomerase from Thermus aquaticus characterized in that the xylose isomerase has the optimal pH of about 7, the stable pH range of from about 6 to 8.5, the optimal temperature of about 95.degree. C. and the molecular weight of about 44,000, and is stabilized with manganese or magnesium. A process for preparing a xylose isomerase comprising transforming a microorganism with a plasmid containing the above gene and a promoter, culturing the transformed microorganism and harvesting the produced xylose isomerase. A process for preparing fructose comprising isomerization of glucose to fructose in the presence of the above xylose isomerase.

Xylose isomerase gene of Thermus aquaticus

Disclosed is a DNA comprising a mucleotide sequence coding for the signal peptide of Bacillus brevis substantially represented by the sequence (I): ##STR1##

Expression vectors containing a Bacillus brevis signal sequence

A novel .beta.-amylase gene is provided. This gene is useful for production of a thermophilic .beta.-amylase obtained from Clostridium thermosulfurogenes on a large scale, especially from genetically engineered microorganisms introduced with this gene. B. subtilis was transformed with a plasmid pNK1 containing the above gene and the transformed B. subtilis cells produced .beta.-amylase having the same thermostability as that of .beta.-amylase obtained from C. thermosulfurogenes.

.beta.-amylase gene

An improved vector for expression in Bacillus brevis having:

Process for expressing genes by Bacillus brevis

A streptothricin-group compound having the formula: ##STR1## in which R represents ##STR2## is disclosed along with methods for producing this compound and a microorganism useful in its production.

Streptothricin-group compounds

A high molecular weight peptide antibiotic designated as AN-7D which is effective as an agent.

High molecular weight antitumor antibiotic AN-7D

A method of cleaning phosphorus-containing waste water by utilizing microorganisms which are capable of accumulating efficiently organic and inorganic phosphorus compounds in themselves. Such microorganisms are found to belong to the Arthrobacter, Micrococcus, Nocardia, Cellulomonas, Oerskovia, Corynebacterium, Brevibacterium and Kurthia genuses.

Method of cleaning phosphorus-containing waste water by microorganisms

Growing community of inventors

Nagoya, Japan

Shigezo Udaka