Sumitomo Chemical Company, Limited

Shigeyasu Nabeshima

Tsuneyuki Nagase

Hideo Hirohara

Satoshi Mitsuda

Masanori Fujimoto

In a process for extracting glucose isomerase from microorganism cells containing glucose isomerase by the so called high pressure release and impact method in which the suspension of the microorganism cells is homogenized for the extraction by releasing the pressure applied on said suspension instantaneously and giving the suspension an impact at very high velocity, the concentration of the suspension containing microorganism cells and the pressure applied on the said suspension are selected within specific ranges, namely from 0.5 to 10 wt. % (on a dry substance base) and from 400 to 700 Kg/cm.sup.2, respectively.

Method for isolation of glucose isomerase

Enzymes are immobilized by a process wherein the carrier used for immobilizing is a hydrophilic pullulan gel having a three-dimensionally reticulated structure obtained by the reaction between pullulan and a bifunctional compound capable of forming an ether linkage with the hydroxyl group contained in the glucose unit of pullulan, or an ionic pullulan gel obtained by the reaction between said hydrophilic pullulan gel and a compound having at one end an ionizable group and at the other end a functional group capable of forming an ether linkage with the hydroxyl group in the pullulan gel in the presence of an alkaline coumpound. Immobilized enzymes prepared according to this process have a high activity and good retention of the activity.

Enzyme immobilization with pullulan gel

Enzyme-immobilization carriers which comprise a macroporous amphoteric ion-exchange resin having a cation-exchange capacity due to carboxymethyl groups of 0.5 meq/g-dry resin or more, an anion-exchange capacity of 1 meq/g-dry resin or more, and a specific surface area of 1 m.sup.2 /g-dry resin or more, the total volume of macropores having a diameter of 100 A to 2000 A being 0.1 cc/g-dry resin or more, and a process for producing said carriers.

Enzyme-immobilization carriers and preparation thereof

A diethylaminoethyl derivative or its salt is reacted with a synthetic resin of a macroporous type having functional groups reactive with said diethylaminoethyl derivative or its salt, having a specific area of at least 1 m.sup.2 /g-dry resin and containing macropores of which the total volume of those with pore sizes from 100 A to 2,000 A is at least 0.1 cc/g-dry resin, in the presence of an alkaline compound to give an enzyme-immobilization carrier which can provide an immobilized enzyme high in activity with good activity retentivity and also large in amount of the immobilized enzyme per unit weight of the carrier.

Enzyme-immobilization carrier and preparation thereof

Non-specific proteases produced by bacteria belonging to the genus Streptomyces are immobilized by adsorption on a specific class of anion exchangers to produce water-insoluble enzymatically active immobilized proteases.

Immobilized proteases

A method for optical resolution of DL-lysine alkyl ester or acid-addition salts thereof by contacting an aqueous solution of the ester or salts thereof with a non-specific protease obtained by culturing bacteria belonging to the genus Streptomyces, e.g. St. griseus, at weakly acidic pH and a temperature not exceeding 60.degree. C until 60 - 90% by weight of L-lysine ester or acid-addition salts thereof takes part in a reaction, and recovering L-lysine or acid-addition salts thereof thus produced by a conventional manner. The protease may be immobilized on an insoluble carrier in advance.

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Ibaraki, Japan

Shigeyasu Nabeshima