Suzuki Motor Corporation

Hamamatsu-photonics K.k.

Shigeru Uchiyama

Takayuki Inoue

Masatoshi Okugawa

Norikazu Sugiyama

Masahiko Hirano

Yoshinori Mizuguchi

Takumi Takano

Hideshi Oishi

Masafumi Oshiro

Kiyoshi Kamiya

Jeremy Cooke

Eiji Inuzuka

Koji Suzuki

Hideji Fujiwake

Keisuke Nozawa

Yuichiro Tani

Hideshi Ohishi

Masahumi Oshiro

Iwayo Nakagawa

Jeremy c/o Hamamatsu Photonics Uk Limited Cooke

Motorcycle

The present invention relates to a microscopic image capturing apparatus having a structure that, in scanning an imageable area of an imaging unit in a predetermined direction in an imaging object area, in which a sample is present, can reliably set a focal point of the imaging unit on each imaging position set inside the imaging object area regardless of the type of focusing actuator. The microscopic image capturing apparatus has a sample setting stage having a sample setting surface that is inclined with respect to a scan plane orthogonal to an optical axis of an objective lens. By moving the sample setting stage along the scan plane such that the distance in the optical axis direction between the imaging unit and the sample setting surface varies monotonously, the focal point position of the imaging unit is adjusted in only one direction along the optical axis of the objective lens.

Microscopic image capturing device

In acquisition of a micro image of a sample by a micro image acquiring unit, when a plurality of image acquiring ranges are set for the sample as an object of image acquisition, a plurality of corresponding focus information are set, and furthermore, when a plurality of partial images acquired by scanning the sample by the micro image acquiring unit include a partial image including mixing of a plurality of image acquiring ranges, the focus information is switched in the middle of scanning of the partial image. With such a structure, even when a plurality of objects are contained in the sample, images of the respective objects can be preferably acquired. Thereby, an image acquiring apparatus, an image acquiring method, and an image acquiring program which are capable of preferably acquiring images of a plurality of objects are realized even when the plurality of objects are contained in a sample.

Image acquiring apparatus, image acquiring method, and image acquiring program

In acquisition of a micro image of a sample S by a micro image acquiring unit, when a plurality of image acquiring ranges are set for the sample S as an object of image acquisition, a plurality of corresponding focus information are set, and furthermore, when a plurality of partial images acquired by scanning the sample S by the micro image acquiring unit include a partial image including mixing of a plurality of image acquiring ranges, the focus information is switched in the middle of scanning of the partial image. With such a structure, even when a plurality of objects are contained in the sample S, images of the respective objects can be preferably acquired. Thereby, an image acquiring apparatus, an image acquiring method, and an image acquiring program which are capable of preferably acquiring images of a plurality of objects are realized even when the plurality of objects are contained in a sample S.

A triple view imaging apparatus is provided for measuring quantitative distribution of material or property in a sample. In the triple view imaging apparatus, an optical system receives an original optical image of the sample, separates the original optical image into at least two secondary optical images having different optical properties from one another, and projects the at least two secondary optical images into a single view angle. A single video camera simultaneously picks up the thus projected plurality of secondary optical images as a single composite image and produces image signals representing the light intensities of the plurality of secondary optical images. An image processor receives the image signals and processes the image signals to obtain final image signals representing a relationship between the image signals for respective ones of the plurality of secondary optical images. An image display receives the calculated final image signals and displays a tertiary optical image based on the calculated final image signals which defines quantitative distribution of material or property in the sample.

Triple view imaging apparatus

Configuration image data representing an external form of an object is stored in advance in a configuration image memory. A two-dimensional image of faint light emitted from the object is detected by a high-sensitivity image pickup means including a two-dimensional photon-counting tube. Faint light image data representing the detected faint light image is accumulated by an adder circuit, and stored in a faint light image memory. The stored faint light image data is superposed on the stored configuration data for each accumulating operation, and based on the superposed image data a superposed image is successivley displayed on a display device.

Image processing apparatus

The present invention relates to an equipment for analyzing the Ca.sup.2+ concentration in the cardiomyocytes, wherein a fluorescent reagent is loaded into the cells to cause intracellular carboxyl groups to be coupled with Ca.sup.2+. The cells are subjected to pulse-excited lights of differing in wavelengths during a vertical blanking period following a predetermined period of time after application of the stimulative signal. The signal causes the cells to generate fluorescent images varying in fluorescence intensity due to the difference in the wavelength of the pulse-excited lights. The resultant images generated are captured by a high-sensitivity television camera and displayed on a video monitor. A plural number of image data are obtained by varying the timing of the stimulating signal with the images stored in image memory. Image data is accessed through a central processing unit which two-dimensionally analyzes the change in the shape of a single cardiomyocyte due to the contraction and the dynamic change in the intracellular Ca.sup.2+ concentration.

Image processing equipment with light source synchronized to blanking

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Hamamatsu, Japan

Shigeru Uchiyama