Japan Agency for Marine-earth Science and Technology

Independent Administrative Institution, Japan Agency for Marine-earth Science and Technology

Japan Marine Science and Technology Center

Kyokuto Pharmaceutical Industrial Co., Ltd.

Shigeru Deguchi

Noriaki Masui

Kaoru Tsujii

Koki Horikoshi

Mikiko Tsudome

Nao Ifuku

Susumu Ito

Keigo Kinoshita

Shunichi Urayama

Kazunori Nagaki

Yasunori Kurosawa

Hiroshi Kitazato

Takuro Nunoura

Takuo Shiraishi

The disclosed invention is device for preparation of an emulsion and a method for producing an emulsion. The method comprises the steps of: (1) causing water and a water-insoluble substance that is to be emulsified to become freely miscible under conditions of temperature and pressure in the vicinity of the vapor-liquid critical point of water; and (2) cooling the product comprised of the water-insoluble substance and water solved each other in the presence of a surfactant to obtain a liquid comprised of the water-insoluble substance dispersed in water or a liquid comprised of water dispersed in the water-insoluble substance. The present invention provides a new device and a new method for producing emulsions with high efficiency without requiring a long period of processing or a great amount of energy.

Method for producing emulsion

The object is to provide a method that enables detection of unknown virus sequences and efficient detection and search of viruses. The method comprises the step of randomly fragmenting an objective double-stranded (ds) RNA to obtain dsRNA fragments; the step of subjecting the obtained dsRNA fragments to a reverse transcription reaction and then performing polymerase chain reaction (PCR) to obtain corresponding DNA fragments; and the step of subjecting the obtained DNA fragments to a sequence analysis operation to determine the sequences. The reverse transcription reaction is preferably started from the 3' ends of the dsRNA fragments.

Method for fragmenting double-stranded RNA and use of the same

A radical polymerization method that enables synthesis, at a high conversion rate, of polymers having a relatively uniform molecular weight and is applicable to various monomers, and an apparatus used for the method. A reaction solution containing monomer and initiator is continuously or intermittently circulated in the apparatus and is heated in a heating-initiation unit to a predetermined temperature simultaneously across a radial direction of a cross section of the flow path whereby the initiator located in a particular volume of the reaction solution is cleaved all at once. The apparatus includes a heating medium production unit, a heating medium-reaction solution mixing unit, a cooling unit, and a recovery unitfor a reaction mixture, all communicatively connected by the flow path and optionally a heating reaction unit, a cooling unit, and a recovery unit, all communicatively connected by the flow path.

Radical polymerization method and polymerization reaction apparatus

The disclosed invention is a method for producing an emulsion, comprising the steps of: (1) causing water and a water-insoluble substance that is to be emulsified to become freely miscible under conditions of temperature and pressure in the vicinity of the vapor-liquid critical point of water; and (2) cooling the product comprised of the water-insoluble substance and water solved each other in the presence of a surfactant to obtain a liquid comprised of the water-insoluble substance dispersed in water or a liquid comprised of water dispersed in the water-insoluble substance. The present invention provides a new method for producing emulsions with high efficiency without requiring a long period of processing or a great amount of energy.

Method for manufacturing emulsion

A cellulose gel medium having good visibility of microbial colonies, a cellulose gel culture substrate for manufacturing the cellulose gel medium, a method for manufacturing the cellulose gel culture substrate, a method for screening cellulase-producing microorganisms or cellulase activity with greater efficiency and rapidity, and a culture substrate, which includes a cellulose gel containing cellulose and water as medium-solidifying components, the cellulose has the viscosity of 12 to 35 mPa&#xb7;S as measured at 26&#xb0; C. with a solution prepared by dissolving the cellulose at a concentration of 2.5 mg/mL in dimethylacetamide containing 8% (W/V) lithium chloride.

Culture substrate comprising cellulose gel, solid medium using same, and cellulase activity assay method using medium

The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel. The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

Solid cellulosic culture medium and process for producing the same

A core sample collector includes a cylinder and a piston, wherein the piston includes a piston body, upper piston rings and lower piston rings, and a sterilizing agent is held in a space defined between the piston, the cylinder, the upper piston ring and the lower piston ring. The sterilizing agent may be an antimicrobial polymeric gel. A core sample is collected by contacting the cylinder against a surface from which the core sample is to be taken, and causing relative movement between the cylinder and the piston such that: (i) the sterilizing agent is applied to the inner peripheral wall surface of the cylinder, (ii) the upper and lower piston rings scrape the sterilizing agent from the inner peripheral wall, and (iii) a core is received in the cylinder.

Core sample collector equipped with sterilizing agent-applying mechanism and method of taking core sample

Disclosed herein is a method of coring a crustal core sample, which comprises using a crustal core sampler equipped with a flow-able coating material-ejecting mechanism for ejecting a flow-able coating material, and ejecting the flow-able coating material composed of a polymer substance having a colloidal particles capturing ability by aggregating function and a water-absorbing polymer substance, from the flow-able coating material-ejecting mechanism of the crustal core sampler thereby coring a crustal core sample in a state coated with the flow-able coating material.

Method of coring a crustal core sample and flow-able coating material for coring a crustal core sample

A method of taking a crustal core sample, wherein the crustal core sample is obtained in a state coated with an antimicrobial polymeric gel formed of a polymer and an inorganic antimicrobial agent dispersed in the polymer. The inorganic antimicrobial agent is a compound containing at least one of silver, zinc or ions thereof. The inorganic antimicrobial agent is carried on a carrier material. The polymer forming the antimicrobial polymeric gel contains a hydrophilic group, and the antimicrobial polymeric gel contains the inorganic antimicrobial agent in a proportion of 0.0001 to 10.0 mass %.

Method of coring crustal core sample, and antimicrobial polymeric gel and gel material used in the method

A method of taking a crustal core sample, wherein the crustal core sample is obtained in a state coated with an antimicrobial polymeric gel formed of a polymer obtained by polymerizing an antimicrobial monomer, preferably a quaternary ammonium salt compound. The polymer preferably contains a component derived from the antimicrobial monomer in a proportion of 1 to 10 mol%.

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Kanagawa, Japan

Shigeru Deguchi