Seikagaku Kogyo Kabushiki Kaisha (seikagaku Corporation)

Seikagaku Corporation

Seikagaku Kogyo Co. Ltd.

Seikagaku Kogyo Kabushiki Kaisha

Hitachi, Ltd.

Nippon Steel Sumitomo Metal Corporation

Seikagu Corporation

Shigenori Tanaka

Hiroshi Tamura

Jun Aketagawa

Shoji Takahashi

Yuko Shibata

Makoto Ohki

Toshio Oda

Kazuhiro Aita

Hiroshi Kanai

Kohei Ueda

Yuichi Hamamura

Yoshihiro Suemune

Akiyoshi Matsumoto

Hiroyasu Furukawa

Kei Imazawa

Maki Aizawa

Chizuko Nakahara

Katsunori Tobisawa

Isamu Momose

Kengo Yoshida

Yusaku Fukaya

Maki Watanabe

Kouichirou Tada

Kenji Inada

A method for work quality control of a worker in work where repetitive operation is performed which includes: a model construction step of statistically constructing, from past path data of the worker, past intermediate quality data on a product to be subjected to the work, and past final quality data on the product to be subjected to the work, a prediction model that receives the path data and the intermediate quality data and outputs the final quality data; a worker position recognition step of recognizing a position of the worker from image data on the work captured; and an unusual worker position determining step of substituting the position of the worker recognized in the worker position recognition step into the model constructed in the model construction step, to determine whether the position of the target worker is a usual one or an unusual one.

Method and apparatus for work quality control

A precoated metal sheet produced by simultaneous multilayer coating, and a process for producing the same, is provided with improved color shading prevention and adhesion. In the precoated metal plate, the centerline average roughness Ra in the coating film interface is not less than 0.3 &#x3bc;m, and the maximum height from the waviness center line in the interface is not more than 50% of the layer thickness. Also provided is (i) a top clear coated metal plate, which comprises a clear layer as an upper layer, has an interfacial centerline average roughness Ra of 0.3 to 0.7 &#x3bc;m and has excellent weathering resistance; and (ii) a precoated metal plate having excellent processability and corrosion resistance, which contains not less than 30% by mass of a rust preventive pigment.

Precoated metal sheet and method of production of precoated metal sheet

A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing areagent to contact with a LAM-containing sample; a method for removing reactivity of LAM with areagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using areagent, which comprises at least a step of removing reactivity of LAM with areagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using areagent, which comprises at least a step of removing reactivity of LAM with areagent by the described-described removing method; and the like.

Method of measuring lipoarabinomannan and application thereof

A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing areagent to contact with a LAM-containing sample, a method for removing reactivity of LAM with areagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using areagent, which comprises at least a step of removing reactivity of LAM with areagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using areagent which comprises at least a step of removing reactivity of LAM with areagent by the described-described removing method; and the like.

A method of eliminating the reactivity of lipoarabinomannan contained in a sample to areagent including at least the step of allowing the sample containing lipoarabinomannan to coexist together with a metal salt or a buffer; and a method of assaying an endotoxin and a method of detecting an endotoxin-associated disease by using the above-described method.

Method of eliminating reactivity of lipoarabinomannan and application of the same

A method for measuring an enzyme reaction to determine an amount of a substance involved in the enzyme reaction, which comprises measuring a time course of a parameter of the enzyme reaction, measuring a time required for the parameter of the enzyme reaction to change from a first threshold value to a second threshold value, and correlating the measured time to an amount of the substance involved in the enzyme reaction.

Method for measuring enzyme reaction

The present invention relates to a process for preparing (1&rarr;3)-&bgr;-D-glucan, comprising subjecting a fungal cell to oxidation degradation under alkaline conditions to release (1&rarr;3)-&bgr;-D-glucan from a cell wall of the cell. (1&rarr;3)-&bgr;-D-Glucan can be prepared easily and efficiently from a cell of a microorganism belonging to the genus Candida. An accurate measurement process having a high reproducibility of (1&rarr;3)-&bgr;-D-glucan can be provided by using the above-described glucan or stabilized glucan into a kit and using it as a standard substance, and a large amount of the (1&rarr;3)-&bgr;-D-glucan having various biological activities can be solubilized and purified from a fungus, such as Candida or the like.

Process for preparing (1&rarr;3)-&bgr;-D-glucan from fungi

The presence of endotoxin in the outer membrane of cell wall of a Gram-negative bacteria is used to determine bacterial contamination in a biological product. The amount of endotoxin present in a biological product is accurately measured without influence of a limulus reaction-activating substance which causes a false-positive reaction, by a method including the steps of: inactivating the limulus reaction-activating substance, if any, in the biological product, by exposing the biological product to a surfactant at a temperature ranging from the surfactant's freezing point to,&deg; C.; and measuring the amount of endotoxin present in the biological product, using a limulus reagent.

Limulus reaction-activating substance, method for inactivating the substance and measuring its amount, and method for measuring limulus reaction

(.fwdarw.)-.beta.- d-glucan binding protein, an antibody recognizing the

An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

Endotoxin stabilizing agent, endotoxin composition and method for

This invention provides (1) a reagent for endotoxin assay which comprises aprotinin and a limulus amebocyte lysate reagent, (2) a kit for endotoxin assay which comprises the limulus amebocyte lysate reagent and a reagent containing aprotinin, (3) a method for assaying endotoxin in a sample using the limulus amebocyte lysate reagent in which aprotinin is added to the lysate reagent and/or the sample, (4) a method for assaying endotoxin in a serine protease-containing sample using the limulus amebocyte lysate reagent in which the sample is allowed to contact with an aprotinin-immobilized insoluble carrier in advance of endotoxin assay, (5) a carrier for pretreating a serine protease-containing sample on which aprotinin is immobilized, (6) a method for inhibiting factor G activation in which aprotinin is added to the limulus amebocyte lysate reagent and (7) a factor G activation inhibitor which comprises aprotinin as an active ingredient. Endotoxin assay can be effected based on the factor C system reaction, without influences of factor G contained in the limulus amebocyte lysate reagent and/or serine proteases contained in samples.

Reagent for endotoxin assay and method for endotoxin assay using the same

Endotoxin (Et) can be specifically assayed by exclusively utilizing the factor C system reaction without being affected by factor G contained in a limulus amebocyte lysate reagent. The present invention provides: (1) a reagent for ET-specific assay which comprises a limulus amebocyte lysate reagent and an alkylglucoside; (2) a method of specifically assaying Et in a specimen using a limulus amebocyte lysate reagent, wherein an alkylglucoside is added to the limulus amebocyte lysate reagent and/or the specimen; and (3) a factor G activation inhibitor composition which comprises an alkylglucoside as an active ingredient capable of inhibiting the activation of factor G in limulus amebocyte by (1.fwdarw.3)-.beta.-D-glucan.

Reagent for endotoxin-specific assay

The present invention provides a reagent for determining (1.fwdarw.3)-.beta.-D-glucan comprising a lysate substantially free from any endotoxin-sensitive factor, which is obtained by contacting limulus amebocyte lysate optionally containing dextran with a polyamide or cellulose insoluble carrier, which makes it possible to rapidly and easily determine at a high accuracy (1.fwdarw.3)-.beta.-D-glucan of mycotic origin contained in the body fluid such as blood or urine.

Growing community of inventors

Tokyo, Japan

Shigenori Tanaka