Honda Motor Co., Ltd.

Thermostable Enzyme Laboratory Co., Ltd.

Shigenobu Mitsuzawa

Satoru Shinkawa

Maiko Tanaka

Daisuke Shibata

Masayuki Machida

Hiroko Hagiwara

Hideaki Koike

Mahoko Kuninaga

Takeshi Ara

Migiwa Takeda

Tomohiro Imai

Maiko Fukuura

Keisuke Nakamura

Tsuyoshi Baba

Minako Onodera

Hiroshi Ono

Hideki Sakai

Masahiro Kimura

Koh Shikata

Yasuhiro Kashima

Keiichi Kimura

A driver support system includes: an acquisition unit that acquires biometric data of a driver of a moving object; an evaluation unit that evaluates a health condition of the driver; and an interactive response unit that executes an interaction operation for interactive response with the driver using an HMI device mounted on the moving object, the evaluation unit being configured to; when estimating based on the biometric data for the driver that the health condition of the driver is poor, instruct the interactive response unit to execute the interaction operation, thereby acquiring a response input, which is a response of the driver to a response facilitating output that facilitates the driver to respond in the interaction operation; and determine the health condition of the driver based on a degree of suitability of the response input to the response facilitating output.

Driver support system, driver support method, and storage medium

To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequencefor protein expression includes: a geneencoding protein; a promoterof the gene, the promoter being linked upstream of the gene; and a cis elementwhose activity is improved by an artificial transcription factor, the cis element being linked further upstream of the promoter. The cis elementis represented by SEQ ID NO: 1.

Base sequence for protein expression and method for producing protein using same

Nucleic acid sequence for protein expression

Anmutant strain characterized in that it is an auxotrophic mutant strain ofstrain AOK27L.

Aspergillus mutant strain

An object of the invention is to provide cellobiohydrolase with high activity. The activity of cellobiohydrolase can be improved by substituting, with alanine, asparagine that is the amino acid at position 62 from the N-terminal of cellobiohydrolase shown in SEQ ID NO: 1, or asparagine at a position corresponding to the position 62 in an amino acid sequence corresponding to the sequence of the cellobiohydrolase shown in SEQ ID NO: 1.

Method for producing highly active cellobiohydrolase and highly active cellobiohydrolase

A saccharifying enzyme composition, by which excellent saccharification performance can be attained even with a low usage thereof, as well as a method for producing a saccharified solution using the same are provided. The saccharifying enzyme composition subjects lignocellulose-based biomass as a substrate to a saccharification treatment. The saccharifying enzyme composition comprises an endoglucanase not containing a cellulose-binding domain, a cellobiohydrolase containing a cellulose-binding domain, and a &#x3b2;-glucosidase containing a cellulose-binding domain.

Saccharifying enzyme composition and method for producing saccharified solution using the same

Provided are anmutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into themutant strain. Themutant strain has been completely or partially deficient in three genes ofstrain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.

Aspergillus mutant strain and transformant thereof

The present invention relates to a polypeptide which has &#x3b2;-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel &#x3b2;-glucosidase enzyme derived from, a polynucleotide encoding the &#x3b2;-glucosidase, an expression vector for expressing the &#x3b2;-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the &#x3b2;-glucosidase can be provided.

&#x3b2;-glucosidase

The present invention relates to a polypeptide which has &#x3b2;-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel &#x3b2;-glucosidase enzyme derived fromcellulolyticus, a polynucleotide encoding the &#x3b2;-glucosidase, an expression vector for expressing the &#x3b2;-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the &#x3b2;-glucosidase can be provided.

Anmutant strain obtained by deleting a ligD gene from an auxotrophic mutant strain ofstrain AOK27L.

Provided is a pretreated product for saccharification, obtained by a pretreatment alone which allows an efficient enzymatic saccharification treatment to thereby obtain a saccharified solution having a uniform quality. A pretreated product for saccharification where ligninis dissociated or a substrateis swollen is obtained by pretreating the substrate. The substratehas 60 to 80% of the pores having a diameter of 10 to 50 nm. In a process for producing the pretreated product for saccharification, a substrate mixture obtained by mixing the substrateand ammonia water is maintained at a predetermined temperature for a predetermined period of time to thereby separate a part of hemicellulosehaving a molecular weight of 7.8&#xd7;10to 2.0&#xd7;10.

Pretreated product of lignocellulosic biomass for saccharification and process for producing the same

A process for producing a saccharified solution, by which xylose can be produced at a high yield from lignocellulosic biomass, is provided. The saccharification is carried out by adding a saccharifying enzyme to a pretreated material for saccharification of the lignocellulosic biomass. As the saccharifying enzyme is added a mixture of a first saccharifying enzyme originated from one or more microorganisms selected from the group consisting of fungi of the genus, the genus, the genus, the genusand the genus, and eubacteria of the genusand the genus, and a second saccharifying enzyme composed of &#x3b2;-xylosidase originated from

Process for producing saccharified solution of lignocellulosic biomass

To provide an apparatus for pretreatment for saccharification easily and continuously applying a treatment and capable of reusing ammonia at low cost. The apparatus has a mixing unit, a first heating unit, a separation unit, a transfer unit, an ammonia water supply unitand an ammonia recovery unit. The apparatus may have the first heat exchanger, a second heat exchangerand a heat supply unitand further has a second heating unit. The apparatus may have a wet grinding unitbetween the heating unitand

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Saitama, Japan

Shigenobu Mitsuzawa