Denki Kagaku Kogyo Kabushiki Kaisha

Shigeki Shigesada

Hironoshin Kitagawa

Yoshiaki Ishimatsu

Shoji Kimura

Toshio Mihara

A process for producing an immobilized enzyme composition comprises simultaneously reacting a non-proteolytic enzyme and a water soluble-multifunctional reagent with a non-hardened granular gelatin in an aqueous medium wherein the reacting is carried out in the presence of a water-soluble protein polymer compound in an amount of from 0.01 to 2 parts by weight relative to one part of the non-proteolytic enzyme. The non-proteolytic enzyme forms a uniform film on the surface of the granular gelatin and the bond between the non-proteolytic enzyme and the granular gelatin is strengthened by the water soluble protein polymer.

Immobilization of enzymes on granular gelatin

Immobilized enzyme compositions for industrial use are produced by reacting an enzyme and/or enzyme-containing microorganism cells with an anion-exchange high molecular substance having a quaternary pyridine ring in the molecule, and then subjecting the reaction products to molding and drying in a molding machine. A polyfunctional crosslinking agent may be added at any stage of the process. The immobilized enzyme compositions thus prepared can be packed in a reactor of an industrial scale for continuous enzyme reaction.

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Sagamihara, Japan

Shigeki Shigesada