Sysmex Corporation

Shigeki Abe

Ryuichiro Ebi

Koki Tajima

Yousuke Tanaka

Junya Inoue

Yasuhiro Otomo

Masanori Kawano

Kazuki Nakabayashi

Harumi Minekawa

Yasumasa Akai

Sachiyo Tada

Yasuyuki Imura

Kazuhiko Takeda

Kei Shirasuna

Disclosed is a cell analysis method comprising: extracting target cells from a population of cells derived from an epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm; classifying the target cells into at least a first group and a second group by difference of amount of DNA; and evaluating a pathology of the epithelial tissue by comparing a ratio of numbers of cells between the first and second groups with a threshold; wherein the threshold varies according to a proportion of the target cells in the population.

Cell analysis method, cell analyzer and sample screening method

There is provided a canceration information providing method which can detect the possibility of cancer in the initial stage with high accuracy. The canceration information providing method for providing information pertaining to canceration of cells includes: acquiring measurement data including first data pertaining to size of a cell nucleus and second data pertaining to size of a cytoplasm for each cell contained in a measurement sample which includes cells collected from epithelial tissue; extracting the measurement data of cells to be analyzed, which are at least some of the cells located toward the basal membrane side of the cells existing in the surface layer in the epithelial tissue, from the measurement data of a plurality of cells in the measurement sample based on the first data and the second data acquired for each cell; and analyzing the extracted measurement data and outputting the information pertaining to the canceration of cells.

Providing cervical or oral mucosa canceration information from measurement of cells located toward the basal membrane side of epithelial tissue

Provided is a canceration information providing method capable of presenting the information related to canceration of the cells with high reliability. A cell in which the amount of DNA is greater than or equal to the amount of DNA of the normal cell in the S period is extracted from a cell group of V&#x2264;N/C ratio&#x2264;V(first counting step). If the number of cells obtained in the first counting step is greater than or equal to the threshold value S1 (S: YES), 'Cancer' is set to a flag. A cell in which the amount of DNA isC is extracted from a cell group of V&#x2264;N/C ratio&#x2264;V(second counting step). A ratio of the number of cells obtained in the second counting step and number of cells obtained in the first counting step is calculated, and 'Cancer' is set to a flagif the ratio is greater than or equal to a threshold value S2 (S: YES). If one of the flagsis &#x201c;Cancer&#x201d; (S: YES), the display of retest necessary is performed.

Cervical cancer information providing method and device

Provided is a canceration information providing method capable of presenting information related to canceration of cells with high reliability. A cell in which an amount of DNA is greater than or equal to an amount of DNA of a normal cell in a S period is extracted from a cell group of V&#x2266;N/C ratio&#x2266;V(first counting step). If a number of cells obtained in the first counting step is greater than or equal to a threshold value S(S: YES), 'Cancer' is set to a flag 1. A cell in which an amount of DNA is 2C is extracted from a cell group of V&#x2266;N/C ratio&#x3c;V(second counting step). A ratio of a number of cells obtained in the first counting step and a number of cells obtained in the second counting step is calculated, and 'Cancer' is set to a flag 2 if the ratio is greater than or equal to a threshold value S(S: YES). If either one of the flags 1, 2 is &#x201c;Cancer&#x201d; (S: YES), retest necessary is displayed.

Canceration information providing method and canceration information providing device

Provided are a cell analyzer, and a cell analysis method. A cell analyzerincludes a measurement devicefor detecting information of each cell from a measurement specimen containing cells harvested from an epithelial tissue, and a data processing devicefor determining appropriateness of the cell harvesting of parabasal cells and acquiring information related to canceration of the cell based on the information detected by the measurement device. The data processing devicedisplays on a display section a dialogue showing 'cell harvesting inappropriate' when determined that the harvesting of parabasal cells is inappropriate.

Cell analyzer and cell analyzing method

A treatment solution for preparing a sample solution for nucleic acid amplification reaction is described. The treatment solution comprises dimethyl sulfoxide and aqueous solvent. The sample solution prepared by treating a biological sample with the treatment solution is used in the amplification reaction of a nucleic acid, whereby the nucleic acid can be efficiently recovered from a biological sample, and the influence of an inhibitor during nucleic acid amplification can be effectively reduced.

Treatment solution for preparing sample solution for nucleic acid amplification reaction and method for detecting nucleic acid by using treatment solution

It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.

Growing community of inventors

Kobe, Japan

Shigeki Abe