Kaneka Corporation

Shigehiko Miyamoto

Jun Tomono

Koji Takahashi

Sotaro Sano

Takaaki Jikihara

Tomohisa Kato

Hozumi Tanaka

Takashi Nishizono

Hiroyuki Hata

Hajime Hiratsuka

Tatsuya Toga

Akiko Kawai

Motonori Okajima

A test device is provided that can comprise: a housing accommodating a chromatography support, wherein the housing comprises: a supporting part that supports a container accommodating a liquid used for chromatography. A method is provided for performing chromatography using the test device.

Chromatographic test device

A primer set includes a terminal primer A including, in its 3'-terminal part, a nucleotide sequence that hybridizes to a 3&#x2032;-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5&#x2032; terminal sides, wherein one of the two polynucleotides includes, in its 3&#x2032;-terminal part, a nucleotide sequence that hybridizes to a 3&#x2032;-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3&#x2032;-terminal part, a nucleotide sequence that hybridizes to a 3&#x2032;-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3&#x2032;-terminal part, a nucleotide sequence that hybridizes to a 3&#x2032;-terminal part of a nucleotide sequence of a N-th target nucleic acid.

Primer set, kit and method for detecting two or more target nucleic acids

A test device includes a housing and a lid. The housing encloses an internal space, has a hole supporting a container accommodating liquid, and includes a perforation/incision part. The lid covers a hole-formed part of the housing. The housing and/or the lid includes a guide that guides the housing and the lid, so that the lid can migrate from the first position to the second position while covering the hole-formed part of the housing. In the first position, the lid covers the hole-formed part of the housing and the container and the container is not incised. During the migration of the lid from the first position to the second position, the container is pushed toward the perforation/incision part by the lid, and the container is incised to leak liquid into the internal space.

Test device

A method for separating a nucleic acid from a specimen includes accommodating a specimen in an accommodation space provided in a nucleic acid-collecting member, transferring a treatment reagent to the nucleic acid-collecting member by connecting a treatment reagent-accommodating container to a treatment reagent-supplying opening of the nucleic acid-collecting member, obtaining a mixture by mixing the specimen and the treatment reagent in a mixing space formed by combining an inner container space of the treatment reagent-accommodating container and the accommodation space of the nucleic acid-collecting member, and pressure-feeding the mixture by reducing a volume of the inner container space of the treatment reagent-accommodating container and discharging the mixture from an mixture-discharging opening of the nucleic acid-collecting member via a carrier that adsorbs the nucleic acid released in the mixture while allowing the mixture to permeate therethrough.

Method for separating nucleic acid from specimen containing nucleic acid and device therefor

A method for producing a dry reagent composition includes preparing a reagent solution including a Good's buffer in a concentration of more than 2.5 mM, an ammonium salt, a drying protection agent, and a nucleic acid amplification enzyme, and drying the reagent solution. A method for suppressing a decrease in enzyme activity during drying includes adding a Good's buffer in a biochemical reagent comprising an ammonium salt and an enzyme prior to drying the enzyme.

Enzyme reaction reagent having dried form and method for preparing the same

An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

Amplified nucleic acid detection method and detection device

Provided is a method for detecting small RNAs in a simple and highly accurate manner. Provided is a nucleic acid detection method including the following steps (a) to (c): (a) carrying out a reverse transcription reaction using a target RNA as a template and a reverse transcription primer having on its 5'-end side a sequence non-complementary to the target RNA to produce a reverse transcription product longer than the target RNA; (b) carrying out a nucleic acid amplification reaction using the reverse transcription product as a template and two primers to produce an amplified double-stranded DNA fragment having a single-stranded region at least at one end; and (c) hybridizing the single-stranded region of the amplified double-stranded DNA fragment to an oligonucleotide probe immobilized on a solid phase.

Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end

A method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by an nucleic acid amplification method, without necessity of special devices are provided. The method for detecting a nucleic acid in a sample comprises: contacting a sample with a dye to react with each other; and observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit for detecting a nucleic acid in a sample comprises: a carrier that holds a dye which can bind to a nucleic acid; a path for passing a sample through the carrier; and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence or absence of a nucleic acid by eye.

Method for detecting nucleic acid, and device or kit

An object of the present invention is to provide methods for amplifying and detecting a nucleic acid that allow efficient hybridization, and devices and kits for use in the methods. The present invention includes amplifying a target nucleic acid into a double-stranded nucleic acid having a single-stranded region at each end, and detecting this nucleic acid. The present invention also provides detection devices and kits that make use of these methods.

Method for amplifying nucleic acid and method for detecting amplified nucleic acid

An object of the present invention is to provide a technique for preparing RNA ready for an enzymatic reaction more easily than conventional techniques. The present invention provides a reagent for RNA extraction from a biological sample which contains an alkali metal salt and a surfactant.

RNA preparation method

The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.

Nucleic acid detection method, and device and kit for use in same

The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.

Growing community of inventors

Hyogo, Japan

Shigehiko Miyamoto