Shanghai Techwell Biopharmaceutical Co., Ltd.

Shidong Liu

Xiaoming Ji

Zhaoli Zhang

Xiusheng Wang

Yi Chen

Zhijun Tang

Zhonghao Zhuo

Xiaoliang Gao

Jing Xu

Xiao Xun Zhang

Bingming He

Guangjun Jiao

Xiao Zhang

Jing Kang

Chunxia Wang

Liangliang Zhou

Jicheng Hu

Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof.

Immobilized cycloaliphatic peptide acyltransferase and preparation method and uses thereof

Provided in the invention is a solvate of a cyclic peptide compound, represented by formula I is the structural formula of the cyclic peptide compound, and, also disclosed are a preparation method for same and uses thereof. 

Solvate of cyclic peptide compound, preparation method for same, and uses thereof

Disclosed is a novel polymorph of a cyclic peptide compound, the structure whereof is represented by formula I; also disclosed are a preparation method for same and uses thereof. 

Crystal of cyclic peptide compound, preparation method for same and uses thereof

Disclosed is a crystalline powder of a cyclic peptide compound, represented by formula I is the structure of same, and, also disclosed are a preparation method for same and uses thereof. 

Crystalline powder of cyclic peptide compound, preparation method for same, and uses thereof

A high purity cyclopeptide crystal has a structure shown by Formula I. R represents H or a cation forming a pharmaceutically acceptable salt. Also disclosed are a preparation method and a use of the high-purity cyclopeptide crystal. 

High-purity cyclopeptide crystal as well as preparation method and use thereof

Provided is a method for preparing a compound represented by Formula I or a salt thereof, whereinF-11899 (FERM BP2635) and/or a mutant strain thereof is cultured in a medium containing amino acid or a salt thereof, an insoluble organic source, and a sugar alcohol, to produce the compound of Formula I or a salt thereof. 

Method for preparing cyclic lipopeptide compound

The method of the preparation of micafungin sodium comprises the step of mixing the weak base and the aqueous solution containing micafungin acid (the structure is illustrated by formula I) or the mixed aqueous solution containing the compound of formula I and organic solvent in order to obtain the sodium salt of micafungin (the structure is illustrated by formula II). 

Preparation method of micafungin sodium

The present invention discloses a crystal of a peptide substance and the preparation method as well as the use thereof. Said crystal B possesses peaks at the following 2&#x3b8; angles in the X-ray diffraction pattern (XRPD): 3.2&#xb1;0.2&#xb0;, 5.4&#xb1;0.2&#xb0;, 6.2&#xb1;0.2&#xb0;, and 9.3&#xb1;0.2&#xb0;.

Crystal of peptide substance as well as the preparation method and use thereof

A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed. 

Process for purifying cyclolipopeptide compounds or the salts thereof

High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid ofof Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

High-yield peptide antibiotics producing strain, preparation method and use thereof

A method for purifying a cyclic lipopeptide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved. 

High yield antibiotics producing fungus strain, preparation method and use thereof are provided. The fungus strain is a mutant derived from, and deposited in CGMCC with the accession number of CGMCC 2933. The preparation method concludes following steps: (a) mixing the culture media ofstrain ATCC 20957 with nitrosoguanidine, and obtaining mixture a; (b) mixing lywallzyme with the mixture a, and obtaining protoplasts; (c) regenerating the protoplasts, and obtaining single clones; and (d) culturing the single clones, then obtaining the mutant strain. This fungus strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable to be used in industry.

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Shanghai, China

Shidong Liu