Arkray, Inc.

Unitika Ltd.

Nipro Corporation

Unitaka Ltd.

Shido Kawase

Keisuke Kurosaka

Hideaki Yamaoka

Koji Sode

Hitoshi Kondo

Akihiko Yamagishi

Masafumi Yao

Munehiko Dombou

Mitsuhiro Hoshijima

Miwa Watanabe

Ryoko Nakatani

Shin-ichi Yokobori

Michie Uchida

The present invention relates to a method for purifying target protein which contains electron transfer protein, from a protein solution containing the target protein, by means of liquid chromatography. The liquid chromatography is performed as follows: First, the protein solution is introduced into a column which is filled with a packing agent, thereby causing the packing agent to bond to the target protein. Then, impurities are removed, and then the target protein is eluted from the packing agent in an eluent which contains a hydroxy-cholate. An example of the target protein is glucose dehydrogenase which contains a protein having glucose dehydrogenation activity. The liquid chromatography is performed as a combination of hydrophobic chromatography and anion exchange chromatography.

Method for purifying protein and glucose dehydrogenase

The present invention relates to glucose dehydrogenase [NAD(P)GDH] using nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a coenzyme, in which the thermal stability and/or the resistance to an organic solvent in the absence of sodium chloride are improved.

Modified glucose dehydrogenase

A process for producing glucose dehydrogenases. This process comprises transferring a DNA containing a sequence represented by SEQ ID NO:1 which encodes an &#x3b1; subunit having a glucose dehydrogenase activity and a &#x3b2; subunit being an electron transfer protein into a microorganism belonging to the genusto thereby construct a transformant, and culturing this transformant so as to allow the production of a first glucose dehydrogenase containing the above-described &#x3b2; subunit and a second glucose dehydrogenase free from the &#x3b2; subunit. The &#x3b1; subunit as described above has a molecular weight of about 60 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions, while the &#x3b2; subunit as described above has a molecular weight of about 43 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions.

Process for producing glucose dehydrogenases

A gene encoding thermostable glucokinase, a recombinant vector comprising this gene, a transformant transformed with the recombinant vector, and a process for producing thermostable glucokinase with the use of the transformant.

Gene for thermostable glucokinase, recombinant vector containing the same, transformant containing the recombinant vector and process for producing thermostable glucokinase using the transformant

A novel sorbitol dehydrogenase having excellent substrate affinity and substrate specificity which can be used to measure D-sorbitol which occurs in human erythrocytes and serum in a slight amount or D-sorbitol or D-fructose contained in foods, a microorganism for producing such an enzyme, and a process for the production of the enzyme using such a microorganism. Also disclosed is provides a method for the measurement of sorbitol using the foregoing sorbitol dehydrogenase and a reagent for the quantitative determination therefor.

Sorbitol dehydrogenase, microorganism for producing same, process for the production thereof, method for the measurement of sorbitol and reagent for the quantitative determination therefor

The present invention provides a gene derived from a thermophilic Bacillus, comprising the nucleotide sequence of SEQ ID No.2 and encoding a thermostable diaphorase comprising the amino acid sequence of SEQ ID No.1, a recombinant vector possessing the gene, a transformant with the recombinant vector and a process for producing the thermostable diaphorase by using the transformant.

Growing community of inventors

Kyoto, Japan

Shido Kawase