Syngenta Participations Ag

Ab Enzymes Gmbh

Shib Sankar Basu

Michael B Lanahan

Joyce Craig

Christopher J Batie

Mark Kinkema

Michael Mahlon Magwire

Wen Chen

Kasimalai Azhakanandam

Thomas Z McNeill

Kristen Kamerath Dang

Sheng Guo

Shengsheng Zhang

Michael L Nuccio

Scott Betts

Jonathan Cohn

Myoung Kim

Rogerio T N Prata

Mark D Kinkema

Xuejun Sean Zhong

Stephanie Winslow

Andrea Nelson

Tricia Charlene Costello

Makoto Ono

Hangsik Moon

Thomas Z Mcneill

The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.

Compositions and methods for protein production

The present invention relates to methods and compositions for identifying, selecting and/or producing a maize plant or maize plant part having increased yield under non-drought conditions, increased yield stability under drought conditions, and/or increased drought tolerance. A maize plant or maize plant part, including any progeny and/or seeds derived from a maize plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided.

Genetic markers associated with drought tolerance in maize

Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration.

Method of measuring alpha amylase

The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency.

Methods of increasing yield and stress tolerance in a plant by decreasing the activity of a trehalose-6-phosphate phosphatase

The invention provides a synthetic phytase polypeptide which encodes an enzymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.

Engineering enzymatically susceptible proteins

The invention provides a synthetic phytase polypeptide which encodes an en/ymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.

Engineering enzymatically susceptible phytases

The present invention relates the nucleotide sequence fromencoding a glucan dikinase enzyme and to methods of use; to modified starch, as well as production and uses thereof. The starch has modified properties of viscosity and a modified phosphate content.

Chlamydomonas glucan dikinase gene, enzyme and modified starch, uses, methods for production thereof

The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are 'self-processing' transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.

Self-processing plants and plant parts

Self processing plants and plant parts

This invention relates to methods of the detection of enzymatically active enzymes in feed added in the pre-pelleting stage. Further, this invention relates to the field of detecting thermotolerant enzymes added to feed in the pre-pelleting stage. In particular, the invention relates to the detection of a phytase enzyme, in particular anphytase or anderived phytase in feed, such as Quantum&#x2122; Phytase.

Extraction methods and assays for feed enzymes

Growing community of inventors

Research Triangle Park, NC, United States of America

Shib Sankar Basu