Chang Gung University

Shiao-Wen Tsai

Min-Hsien Wu

Yen-Ting Liu

Heng-Liang Liu

Tung-Ming Pan

Chao-Wen Lin

Chun-Li Lin

Kung-Yuan Chang

An apparatus for high-throughput cell culture with mechanical compression stimulation includes a cell culture vessel and a fluid pressure supply unit. The cell culture vessel includes at least one culture chamber, at least one pressure chamber disposed above the culture chamber, a membrane disposed between the culture chamber and the pressure chamber, and at least one pressurizing member that projects downwardly from the membrane into the culture chamber. The fluid pressure supply unit is connected fluidly to the pressure chamber, and has a fluid pressure supply device to supply a pressurized fluid to the pressure chamber so as to deform the membrane and move the pressurizing member, and a control device that is adapted to vary a pressure of the pressurized fluid in the pressure chamber.

Apparatus for high-throughput cell culture with mechanical compression stimulation

A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60&#xb0; C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 &#x3bc;l of 98.7 wt % of APS and 50 &#x3bc;l of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 &#x3bc;l of 1&#xd7;PBS buffer solution, uniformly mixing same, adding 100 &#x3bc;l of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37&#xb0; C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTiO; and taking a measurement.

Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes

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Tao-Yuan, Taiwan

Shiao-Wen Tsai