Midwest Research Institute

Alliance for Sustainable Energy, LLC

Shi-You Ding

Michael E Himmel

William S Adney

Todd B Vinzant

Stephen Robert Decker

Yat-Chen Chou

John O Baker

Suzanne Lantz McCarter

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes fromdemonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase fromand tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-nativehost. In a specific example, the specific performance of the formulation using purified recombinant Cel7A fromexpressed inis increased by more than 200% when compared to a formulation using purified Cel7A from

[object Object]

The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized fromand, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Thermal tolerant avicelase from Acidothermus cellulolyticus

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes fromdemonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase fromand tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-nativehost. In a specific example, the specific performance of the formulation using purified recombinant Cel7A fromexpressed inis increased by more than 200% when compared to a formulation using purified Cel7A from

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as Gux1. Gux1 has been isolated and characterized from. The invention further provides recombinant forms of the identified Gux1. Methods of making and using Gux1 polypeptides, including fusions, variants, and derivatives, are also disclosed.

The invention provides a thermal tolerant mannanase that is a member of the glycoside hydrolase family. The invention further discloses this mannanase as ManA. ManA has been isolated and characterized from. The invention further provides recombinant forms of the identified ManA. Methods of making ManA polypeptides, including fusions, variants, and derivatives, are also disclosed. Methods of using mannanase A, including for the processing of food and for use in food stuffs as bulking agents and the like, are also disclosed.

Thermal tolerant mannanase from acidothermus cellulolyticus

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized fromThe invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.

Growing community of inventors

Golden, CO, United States of America

Shi-You Ding